日本静冈县和长野县蜱虫及野生啮齿动物中埃立克体属物种和“新立克次体候选种”的分子鉴定
Molecular identification of Ehrlichia species and 'Candidatus Neoehrlichia mikurensis' from ticks and wild rodents in Shizuoka and Nagano Prefectures, Japan.
作者信息
Naitou Hirotaka, Kawaguchi Daizoh, Nishimura Yuusaku, Inayoshi Megumi, Kawamori Fumihiko, Masuzawa Toshiyuki, Hiroi Midori, Kurashige Hideaki, Kawabata Hiroki, Fujita Hiromi, Ohashi Norio
机构信息
Laboratory of Environmental Microbiology, Institute for Environmental Sciences, School of Pharmaceutical Sciences, University of Shizuoka and COE Program in the 21st Century, Shizuoka 420-8637, Japan.
出版信息
Microbiol Immunol. 2006;50(1):45-51. doi: 10.1111/j.1348-0421.2006.tb03769.x.
A total of 293 ticks and 111 wild rodents that were collected in Shizuoka and Nagano Prefectures, Japan, were examined for infection of Ehrlichia species and 'Candidatus Neoehrlichia mikurensis.' The 16S rDNA or the omp-1 gene of these bacterial DNAs were detected from the spleens of tick-inoculated mice (5 positive/total 29 mice) or from the spleens of wild rodents (25 positive/total 111 rodents) by PCR amplifi-cation. Sequencing of the 16S rDNA revealed Ehrlichia spp. from the 5 tick-inoculated mice and 8 wild rodents, and 'Candidatus N. mikurensis' from 17 wild rodents. The data suggest the presence of additional genetic variants, and potential vectors and/or reservoirs for these bacteria in central Japan.
对在日本静冈县和长野县采集的293只蜱虫和111只野生啮齿动物进行了埃立克体属细菌和“米库新立克次体（Candidatus Neoehrlichia mikurensis）”感染情况的检测。通过PCR扩增，从接种蜱虫的小鼠脾脏（29只小鼠中5只为阳性）或野生啮齿动物的脾脏（111只啮齿动物中25只为阳性）中检测到这些细菌DNA的16S rDNA或omp - 1基因。16S rDNA测序显示，在5只接种蜱虫的小鼠和8只野生啮齿动物中发现了埃立克体属细菌，在17只野生啮齿动物中发现了“米库新立克次体”。数据表明在日本中部存在这些细菌的其他遗传变体以及潜在的传播媒介和/或宿主。
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