Eldridge M G, Dailey H A
University of Georgia.
Biotechniques. 1992 Jun;12(6):848-53.
Murine erythroleukemia (MEL) cells are frequently employed to study both cell growth and erythroid differentiation. Although these cells are easily cultured and induced to differentiate, they are routinely maintained in a medium that contains 10%-15% fetal bovine serum. Because of the variability between different lots and the cost of serum, it was desirable to define a serum-free medium in which to culture MEL cells. In the present work, a totally serum-free, defined medium is described that supports both normal cell growth and dimethyl sulfoxide induced differentiation in the two MEL cell lines examined (DS-19 and 270). A variety of hormones and biological compounds are examined in this medium to determine their effects on growth and differentiation. This medium does not support the growth of the mouse hepatoma cell line.
小鼠红白血病（MEL）细胞常用于研究细胞生长和红细胞分化。尽管这些细胞易于培养且能被诱导分化，但它们通常在含有10%-15%胎牛血清的培养基中维持培养。由于不同批次血清之间存在差异以及血清成本问题，因此需要确定一种无血清培养基来培养MEL细胞。在本研究中，描述了一种完全无血清的限定培养基，该培养基能支持所检测的两种MEL细胞系（DS-19和270）的正常细胞生长以及二甲基亚砜诱导的分化。在该培养基中检测了多种激素和生物化合物，以确定它们对生长和分化的影响。这种培养基不支持小鼠肝癌细胞系的生长。
