Hrabák A, Bajor T, Csuka I
Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Budapest, Hungary.
Inflamm Res. 2006 Jan;55(1):23-31. doi: 10.1007/s00011-005-0004-6.
The effects of various inflammatory stimuli on the alternative arginine metabolic pathways in mouse and rat peritoneal macrophages were investigated in vitro and compared.
Mice and rats were injected i. p. with thioglycollate, carrageenan, casein, BCG and Newcastle Disease Virus (NDV) vaccines.
Peritoneal macrophages were isolated from untreated and treated animals. The activities of nitric oxide synthase (NOS) II and arginase were measured and expressions were followed by Western blotting. The uptake of arginine and nitrite formation of macrophages were also measured.
Inflammatory stimuli increased the NO production and the expression and activity of both NOS II and arginase in mice in vitro. On the contrary, the same treatments changed the expression and activity of NOS II only, but not those of arginase in rats. The most marked effects on NO metabolism were produced by casein and NDV treatments.
The activity and expression of NOS II and arginase can be stimulated in peritoneal macrophages in vitro by injecting inflammatory agents into the peritoneal cavity. A markedly different response in arginine metabolism was observed in mouse and rat macrophages. Casein treatment was a potent inducer for both enzymes. NDV vaccines induced mainly NOS II, while thioglycollate induced arginase.
在体外研究并比较了多种炎症刺激对小鼠和大鼠腹腔巨噬细胞中精氨酸代谢替代途径的影响。
给小鼠和大鼠腹腔注射巯基乙酸盐、角叉菜胶、酪蛋白、卡介苗和新城疫病毒（NDV）疫苗。
从未经处理和经处理的动物中分离出腹腔巨噬细胞。测量一氧化氮合酶（NOS）II和精氨酸酶的活性，并通过蛋白质印迹法追踪其表达。还测量了巨噬细胞对精氨酸的摄取和亚硝酸盐的形成。
炎症刺激在体外增加了小鼠体内一氧化氮的产生以及NOS II和精氨酸酶的表达与活性。相反，相同处理仅改变了大鼠体内NOS II的表达与活性，而未改变精氨酸酶的表达与活性。酪蛋白和NDV处理对一氧化氮代谢产生的影响最为显著。
通过向腹腔注射炎症因子，可在体外刺激腹腔巨噬细胞中NOS II和精氨酸酶的活性与表达。在小鼠和大鼠巨噬细胞中观察到精氨酸代谢存在明显不同的反应。酪蛋白处理是这两种酶的有效诱导剂。NDV疫苗主要诱导NOS II，而巯基乙酸盐诱导精氨酸酶。
