马CCL11诱导嗜酸性粒细胞细胞骨架重组和激活。
Equine CCL11 induces eosinophil cytoskeletal reorganization and activation.
作者信息
Weston M C, Collins M E, Cunningham F M
机构信息
Department of Veterinary Basic Sciences, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hertfordshire AL9 7TA, UK.
出版信息
Inflamm Res. 2006 Jan;55(1):46-52. doi: 10.1007/s00011-005-0007-3.
OBJECTIVES
To assess the biological effects of purified recombinant equine CCL11 on equine eosinophil function.
METHODS
Following stimulation of eosinophils from normal horses, the polymerised form of actin was measured by flow cytometry using fluorescently labelled phalloidin. Migration was determined in a 96 well plate chemotaxis assay using 8 microm pore membranes, and adherence of eosinophils to serum-coated plastic was assessed using a colorimetric assay for eosinophil peroxidase. Superoxide generation was measured by the reduction of cytochrome C in a colorimetric assay.
RESULTS
Equine CCL11 induced significant (p < 0.001), concentration-dependent actin polymerisation and migration of equine eosinophils. Stimulation with CCL11 did not induce significant adherence to serum coated plastic, or superoxide production.
CONCLUSIONS
Equine CCL11 stimulates cytoskeletal reorganization and migration of equine eosinophils, suggesting that it may be involved in the regulation of eosinophil trafficking in horses.
目的
评估纯化的重组马CCL11对马嗜酸性粒细胞功能的生物学效应。
方法
用荧光标记的鬼笔环肽通过流式细胞术测量正常马嗜酸性粒细胞受到刺激后肌动蛋白的聚合形式。在使用8微米孔径膜的96孔板趋化试验中测定迁移情况，并使用嗜酸性粒细胞过氧化物酶比色法评估嗜酸性粒细胞对血清包被塑料的黏附情况。通过比色法中细胞色素C的还原测定超氧化物的生成。
结果
马CCL11诱导马嗜酸性粒细胞发生显著（p < 0.001）的、浓度依赖性的肌动蛋白聚合和迁移。CCL11刺激未诱导对血清包被塑料的显著黏附或超氧化物产生。
结论
马CCL11刺激马嗜酸性粒细胞的细胞骨架重组和迁移，表明它可能参与马嗜酸性粒细胞运输的调节。
Zotero 插件