Yang Mao-bin, Ding Yi, Jiang Jun-qiang, Deng Hui, Tian Hua
Dept of Endodontics, West China College of Stomatology, Sichuan University, Chengdu, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2005 Dec;23(6):467-70.
To evaluate the effect of the pulsed Nd: YAG laser irradiation on the survival rate of human penodontal ligament fibroblast (PDLF) at different power and irradiation time settings, and to compare the morphological change of cells before and after irradiation.
The cells were cultured and placed into the 96-well tissue culture plates. Cells were divided into groups according to the irradiation time (10 s, 20 s, 40 s, 60 s) and the output energy (0.5 W, 1.0 W, 1.5 W, 2.0 W, 2.5 W). After the irradiation, the cell number were counted and the survival rates were calculated. The correlation between the survival rate and irradiation time as well as power output was analyzed.
No significant reduction of survival rates were found in 10 s group, as well as in 20 s group with 0.5 W, 1.0 W setting. The increase of the power output and increase of the irradiation time caused significant reduction of cell survival rate (P <0.05). The irradiation time was more relevant to this reduction than the power output.
It indicates that the laser irradiation may cause damage to PDLF and decrease the cell survival rate. The irradiation time is more related to this effect than the power output.
评估不同功率和照射时间设置下的脉冲Nd:YAG激光照射对人牙周膜成纤维细胞（PDLF）存活率的影响，并比较照射前后细胞的形态变化。
培养细胞并将其置于96孔组织培养板中。根据照射时间（10秒、20秒、40秒、60秒）和输出能量（0.5瓦、1.0瓦、1.5瓦、2.0瓦、2.5瓦）将细胞分组。照射后，计数细胞数量并计算存活率。分析存活率与照射时间以及功率输出之间的相关性。
10秒组以及0.5瓦、1.0瓦设置的20秒组中未发现存活率有显著降低。功率输出的增加和照射时间的延长导致细胞存活率显著降低（P<0.05）。照射时间比功率输出更与这种降低相关。
表明激光照射可能对PDLF造成损伤并降低细胞存活率。照射时间比功率输出更与这种效应相关。
Zotero 插件