基质金属蛋白酶3基因功能性（-1171 5A→6A）多态性作为男性槟榔使用者口腔黏膜下纤维化的危险因素。
The functional (-1171 5A-->6A) polymorphisms of matrix metalloproteinase 3 gene as a risk factor for oral submucous fibrosis among male areca users.
作者信息
Tu H-F, Liu C-J, Chang C-S, Lui M-T, Kao S-Y, Chang C-P, Liu T-Y
机构信息
Department of Dentistry, I-Lan Hospital, I-Lan, Taiwan.
出版信息
J Oral Pathol Med. 2006 Feb;35(2):99-103. doi: 10.1111/j.1600-0714.2006.00370.x.
BACKGROUND
Insertion/deletion (-1171 5A-->6A) polymorphisms in the promoter region of matrix metalloproteinase 3 (MMP3) gene result in different transcriptional activities. MMP3 is able to degrade collagens types II, V, IX, and X, and other extracellular matrix. The functional promoter polymorphism of MMP3 has been related to the susceptibility in some inflammatory diseases and metastasis of cancers.
METHODS
Oral submucous fibrosis (OSF) and oral squamous cell carcinoma (OSCC) are prevalent among Asian areca users. In this study, genomic DNA obtained from the blood of OSCC (n = 150), OSF (n = 71), and control non-diseased areca user (n = 98) in male were subjected to polymerase chain reaction (PCR)-based genotyping of MMP3.
RESULTS
The 5A genotype in MMP3 promoter was observed more frequently in OSF group than in control group (P = 0.01). No significant difference was noted between OSCC and control groups on the 5A genotype frequency (P = 0.18). No association was found between 5A genotype in MMP3 promoter and site or lymph node metastasis and stage of OSCC.
CONCLUSION
The results indicated that the 5A genotype of MMP3 promoter was associated with the risk of OSF but not OSCC.
背景
基质金属蛋白酶3（MMP3）基因启动子区域的插入/缺失（-1171 5A→6A）多态性导致不同的转录活性。MMP3能够降解II型、V型、IX型和X型胶原蛋白以及其他细胞外基质。MMP3的功能性启动子多态性与某些炎症性疾病的易感性和癌症转移有关。
方法
口腔黏膜下纤维化（OSF）和口腔鳞状细胞癌（OSCC）在亚洲槟榔使用者中很常见。在本研究中，对从男性OSCC患者（n = 150）、OSF患者（n = 71）和对照非患病槟榔使用者（n = 98）的血液中获得的基因组DNA进行基于聚合酶链反应（PCR）的MMP3基因分型。
结果
MMP3启动子中的5A基因型在OSF组中的观察频率高于对照组（P = 0.01）。OSCC组和对照组在5A基因型频率上无显著差异（P = 0.18）。未发现MMP3启动子中的5A基因型与OSCC的部位、淋巴结转移及分期之间存在关联。
结论
结果表明，MMP3启动子的5A基因型与OSF风险相关，但与OSCC风险无关。
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