Ramírez de Arellano E, Aznar J, Pascual A
Departamento de Microbiología, Hospital Universitario Virgen Macarena, Sevilla.
Enferm Infecc Microbiol Clin. 1992 Feb;10(2):103-6.
To compare and evaluate four different rapid methods of respiratory syncytial virus antigen detection with the isolation of the virus in cell culture.
Ninety-eight nasopharyngeal swabs were studied, for respiratory syncytial virus isolation in Hep-2 cell line and for RSV antigen detection using direct immunofluorescence and three ELISA methods: Abbot RSV EIA (ELISA-1), RSV Antigen Detection System (ELISA-2) and Directigen RSV (Directigen).
Of all 98 samples studied using the five mentioned methods, 42 (43%) were positive for respiratory syncytial virus. The most sensitive methods were ELISA-1 (82%) and immunofluorescence (82%). The most specific ones were direct immunofluorescence (86%) and Directigen (84%). The higher percentage of concordant positive results with respiratory syncytial virus culture were direct immunofluorescence (84%) and Directigen (82%).
Rapid diagnostic methods for respiratory syncytial virus infection can not substitute the isolation method using cell line culture. We believe that respiratory syncytial virus isolation in cell culture combined direct with direct immunofluorescence or Directigen methods are the first choice diagnostic techniques for achieve a higher number of positive results in respiratory syncytial virus infected patients.
比较和评估四种不同的呼吸道合胞病毒抗原快速检测方法与病毒在细胞培养中的分离方法。
对98份鼻咽拭子进行研究，用于在Hep-2细胞系中分离呼吸道合胞病毒，并使用直接免疫荧光法和三种酶联免疫吸附测定（ELISA）方法检测呼吸道合胞病毒抗原：雅培呼吸道合胞病毒酶免疫测定（ELISA-1）、呼吸道合胞病毒抗原检测系统（ELISA-2）和直接免疫检测呼吸道合胞病毒（Directigen）。
使用上述五种方法研究的所有98份样本中，42份（43%）呼吸道合胞病毒呈阳性。最敏感的方法是ELISA-1（82%）和免疫荧光法（82%）。最特异的方法是直接免疫荧光法（86%）和Directigen（84%）。与呼吸道合胞病毒培养结果一致性阳性率较高的是直接免疫荧光法（84%）和Directigen（82%）。
呼吸道合胞病毒感染的快速诊断方法不能替代细胞系培养的分离方法。我们认为，细胞培养中呼吸道合胞病毒分离结合直接免疫荧光法或Directigen方法是在呼吸道合胞病毒感染患者中获得更多阳性结果的首选诊断技术。
