Arayne M Saeed, Sultana Najma, Siddiqui Farhan Ahmed
Department of Chemistry, University of Karachi, Karachi-75270, Pakistan.
Pak J Pharm Sci. 2005 Jan;18(1):58-62.
A simple and rapid high-performance liquid chromatographic (HPLC) method for the determination and quantification of meloxicam has been developed. The chromatographic system consisted of a Shimadzu LC-10 AT VP pump, SPD-10 AV VP UV/ visible detector, and a CBM-102 Bus Module integrator. Separation was achieved on the micro Bondapak 125 A C18 10 microm column at room temperature. The sample was introduced through an injector valve with a 10 microl sample loop. Methanol:water (70:30 v/v) was used as mobile phase, with flow rate 2 ml/minutes. pH was adjusted to 2.6 with phosphoric acid. U.V detection was performed at 230 nm. The results obtained showed a good agreement with the declared content. Recovery values of meloxicam in tablets (in Melfax 15 mg tablets) were from 99.27 % to 100.06 %. Piroxicam was used as an internal standard. The proposed method is rapid, accurate and selective; it may be used for the quantitative analysis of meloxicam from raw materials, in bulk drugs and other dosage formulations.
已开发出一种用于测定和定量美洛昔康的简单快速的高效液相色谱（HPLC）方法。色谱系统由岛津LC-10 AT VP泵、SPD-10 AV VP紫外/可见检测器和CBM-102总线模块积分仪组成。在室温下，于微Bondapak 125 A C18 10微米柱上实现分离。通过带有10微升进样环的进样阀进样。甲醇:水（70:30 v/v）用作流动相，流速为2毫升/分钟。用磷酸将pH调节至2.6。在230纳米处进行紫外检测。所得结果与申报含量吻合良好。美洛昔康在片剂（美洛昔康15毫克片剂）中的回收率为99.27%至100.06%。吡罗昔康用作内标。所提出的方法快速、准确且具选择性；可用于对原料药、散装药物及其他剂型中美洛昔康的定量分析。
