Munker R, Greither L, Darsow M, Pander S, Wilmanns W
Medizinische Klinik III im Klinikum Grosshadern der LMU, Munich, Federal Republik of Germany.
Ann Hematol. 1992 Jul;65(1):50-2. doi: 10.1007/BF01715127.
We report here that tumor necrosis factor alpha (TNF) induces peculiar cytoplasmic vesicles in the human erythromyeloid leukemia cell line K 562, sensitized to the cytotoxic action of TNF by a treatment with the inhibitor of transcription actinomycin D. These vesicles are well delineated ultrastructurally. The formation of these vesicles is characteristic for the combination of actinomycin D with TNF and precedes the changes of apoptosis and cellular disintegration. These vesicles correspond to an intermediate step in the cytotoxicity caused by TNF and may indicate that reactive metabolites are involved in the mechanism of action of TNF.
我们在此报告，肿瘤坏死因子α（TNF）在经转录抑制剂放线菌素D处理而对TNF细胞毒性作用敏感的人红髓白血病细胞系K 562中诱导形成特殊的细胞质囊泡。这些囊泡在超微结构上界限清晰。这些囊泡的形成是放线菌素D与TNF联合作用的特征性表现，且先于细胞凋亡和细胞解体的变化。这些囊泡对应于TNF引起的细胞毒性作用中的一个中间步骤，可能表明活性代谢产物参与了TNF的作用机制。
