Takasaka Tomokazu, Goya Nobuyuki, Ishida Hideki, Tanabe Kazunari, Toma Hiroshi, Fujioka Tomoaki, Omori So, Zheng Huai-Ying, Chen Qin, Nukuzuma Souichi, Kitamura Tadaichi, Yogo Yoshiaki
Department of Urology, Faculty of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.
Department of Urology, Tokyo Women's Medical University, Tokyo, Japan.
J Gen Virol. 2006 Feb;87(Pt 2):303-306. doi: 10.1099/vir.0.81368-0.
To clarify the stability of the BK polyomavirus (BKPyV) genome in renal transplant (RT) recipients, three to five complete BKPyV genomes from each of six RT recipients with surviving renal allografts were molecularly cloned. The complete sequences of these clones were determined and compared in each patient. No nucleotide difference was detected among clones in two patients, and a few nucleotide variations were found among those in four patients. In each of these patients a parental sequence (usually the major sequence), from which variant sequences (usually minor sequences) with nucleotide substitutions would have been generated, were identified. A comparison between the parental and variant sequences in each patient identified a single nucleotide substitution in each variant sequence. From these findings, it was concluded that the genome of BKPyV is stable in RT recipients without nephropathy, with only minor nucleotide substitutions in the coding region.
为阐明BK多瘤病毒（BKPyV）基因组在肾移植（RT）受者中的稳定性，对6名肾移植存活受者每人的3至5个完整BKPyV基因组进行了分子克隆。测定并比较了每位患者这些克隆的完整序列。两名患者的克隆之间未检测到核苷酸差异，四名患者的克隆之间发现了一些核苷酸变异。在这些患者中，均鉴定出一个亲本序列（通常是主要序列），核苷酸替代的变异序列（通常是次要序列）可能由此产生。比较每位患者的亲本序列和变异序列，发现每个变异序列中有一个单核苷酸替代。从这些发现得出结论，在没有肾病的RT受者中，BKPyV基因组是稳定的，编码区只有少量核苷酸替代。
