Li Y, Wang S, Zhong N
Department of Paediatrics, 202 Hospital, Shenyang, People's Republic of China.
Biomed Chromatogr. 1992 Jul-Aug;6(4):191-3. doi: 10.1002/bmc.1130060407.
A reversed phase high performance liquid chromatographic method for the simultaneous determination of pseudouridine (PU) and creatinine (Cr) in urine is described. The mobile phase was 0.01 mol phosphate buffer (pH 6.1) containing 2.5 mmol octanesulphonic acid as the ion pairing agent. UV detection was set at 250 nm. Variation in pH value affected the retention time of PU and Cr significantly; Their separation from interfering peaks was also affected. The recoveries of PU and Cr were 89.93% and 90.35%, respectively. The standard deviation of the method for PU was 48.69 +/- 0.063 (nmol/mumol Cr, mean +/- SD, n = 5). The urine samples from 233 normal children of different ages and 119 patients with leukaemia were analysed by this method. The normal reference value was appraised by comparison with the percentage of immature cells in the bone marrow. The results showed that the sensitivity of the method was 94.12%, the specificity was 95.86%, the accuracy was 95.50%, the positive predictive value was 82.05% and the negative predictive value was 98.78%. The method can be used to evaluate the state of the leukaemia, and to monitor the effect of treatment.
描述了一种反相高效液相色谱法，用于同时测定尿液中的假尿苷（PU）和肌酐（Cr）。流动相为含有2.5 mmol辛烷磺酸作为离子对试剂的0.01 mol磷酸盐缓冲液（pH 6.1）。紫外检测设定在250 nm。pH值的变化对PU和Cr的保留时间有显著影响；它们与干扰峰的分离也受到影响。PU和Cr的回收率分别为89.93%和90.35%。该方法测定PU的标准差为48.69 +/- 0.063（nmol/μmol Cr，平均值 +/- 标准差，n = 5）。用该方法分析了233名不同年龄正常儿童和119名白血病患者的尿液样本。通过与骨髓中未成熟细胞的百分比进行比较来评估正常参考值。结果表明，该方法的灵敏度为94.12%，特异性为95.86%，准确度为95.50%，阳性预测值为82.05%，阴性预测值为98.78%。该方法可用于评估白血病状态，并监测治疗效果。
