Spaulding S W, Burrow G N
Endocrinology. 1975 Apr;96(4):1018-21. doi: 10.1210/endo-96-4-1018.
PGE1, like TSH, can increase cAMP-dependent protein kinase activity in calf thyroid slices. The intracellular levels of cAMP produced by either of these agents alone appeared to correlate well with the degree of kinase activation. PG synthesis did not appear to be necessary for TSH action in this system, since indomethacin, and inhibitor of prostaglandin synthesis, did not alter either cAMP levels or kinase activity in slices incubated with TSH. Both the cAMP level and kinase activity rose when a submaximally effective dose of TSH was added to a maximal dose of PGE1. However, neither the cAMP levels nor the kinase activity produced by a maximal dose of TSH was affected by the addition of PGE1.
与促甲状腺激素（TSH）一样，前列腺素E1（PGE1）可增加小牛甲状腺切片中依赖环磷酸腺苷（cAMP）的蛋白激酶活性。单独使用这两种物质中的任何一种所产生的细胞内cAMP水平似乎与激酶激活程度密切相关。在该系统中，前列腺素合成似乎并非TSH发挥作用所必需，因为前列腺素合成抑制剂吲哚美辛并不会改变与TSH一起孵育的切片中的cAMP水平或激酶活性。当向最大剂量的PGE1中加入次最大有效剂量的TSH时，cAMP水平和激酶活性均升高。然而，加入PGE1并不会影响最大剂量TSH所产生的cAMP水平或激酶活性。
