Nizański Wojciech
Department and Clinic of Reproduction, Ruminants Diseases and Animal Health Protection, Faculty of Veterinary Medicine, University of Agriculture, pl. Grunwaldzki 49, 50-366 Wrocław, Poland.
Theriogenology. 2006 Jul 15;66(2):470-83. doi: 10.1016/j.theriogenology.2006.01.001. Epub 2006 Jan 24.
One hundred fifty-two bitches of seven breeds were vaginally inseminated with fresh or frozen-thawed semen of 10 stud dogs of respective breeds. The semen was supplemented with prostatic fluid before insemination. In experiment 1 bitches of each breed were randomly assigned to three treatment groups, consisting of 29 females (group 1), 33 females (group 2) and 32 females (group 3). In group 1 bitches were inseminated into vagina with fresh semen using a bovine infusion pipette. In group 2 bitches were inseminated into vagina with fresh semen using the Osiris catheter. In group 3 bitches were inseminated with frozen-thawed semen with the Osiris catheter. The number of sperms in each insemination dose was adjusted to 300 x 10(6). In experiment two bitches were randomly assigned to two treatment groups, consisting of 30 females (group A) and 28 females (group B). In group A bitches were inseminated with fresh semen, whereas in group B with frozen-thawed semen. Osiris catheter was used in both groups. The total number of sperms was adjusted to provide 250 x 10(6) of progressively motile spermatozoa in each insemination dose. In experiment 1 the pregnancy rates/whelping rates were 86.2/82.8%, 81.8/81.8% and 59.4/59.4% for groups 1, 2 and 3, respectively. The differences between group 1 and 3 were statistically significant (p < 0.05). The litter sizes at birth/litter sizes at weaning were 5.8+/-2.3/5.4+/-2.0, 6.3+/-1.4/5.7+/-1.0 and 3.9+/-1.2/3.5+/-1.5 in groups 1, 2 and 3, respectively. The litter size at birth and at weaning was reduced (p < 0.05) when frozen-thawed semen was used for insemination (group 3). There were not significant (p > 0.05) differences in the litter size between groups 1 and 2. In experiment 2 pregnancy rates/whelping rates and litter sizes at birth/litter sizes at weaning were 86.7/86.7%, 60.7/57.1% (p < 0.05) and 6.1+/-1.6/5.7+/-1.7, 4.0+/-1.4/3.8+/-1.4 (p < 0.05) in groups A and B, respectively. This study shows that results of AI with a fresh semen using a bovine infusion pipette and the Osiris catheter are equivalent. The results of the use of the Osiris catheter for vaginal insemination of frozen-thawed dog semen extended with prostatic fluid after thawing are not encouraging. The pregnancy rate, whelping rate and litter size are reduced when frozen-thawed, prostatic fluid-supplemented semen is vaginally deposited using the Osiris catheter.
将七个品种的152只母犬用各自品种的10只种公犬的新鲜或冻融精液进行阴道输精。输精前精液中添加了前列腺液。在实验1中，每个品种的母犬被随机分为三个处理组，分别为29只母犬（第1组）、33只母犬（第2组）和32只母犬（第3组）。在第1组中，使用牛用输精滴管将新鲜精液输入母犬阴道。在第2组中，使用奥西里斯导管将新鲜精液输入母犬阴道。在第3组中，使用奥西里斯导管将冻融精液输入母犬阴道。每次输精剂量中的精子数量调整为300×10⁶。在实验2中，母犬被随机分为两个处理组，分别为30只母犬（A组）和28只母犬（B组）。A组母犬用新鲜精液输精，而B组母犬用冻融精液输精。两组均使用奥西里斯导管。每次输精剂量中的精子总数调整为在每次输精剂量中提供250×10⁶的进行性运动精子。在实验1中，第1、2和3组的妊娠率/产仔率分别为86.2/82.8%、81.8/81.8%和59.4/59.4%。第1组和第3组之间的差异具有统计学意义（p<0.05）。第1、2和3组出生时的窝仔数/断奶时的窝仔数分别为5.8±2.3/5.4±2.0、6.3±1.4/5.7±1.0和3.9±1.2/3.5±1.5。当使用冻融精液输精时（第3组），出生时和断奶时的窝仔数减少（p<0.05）。第1组和第2组之间的窝仔数没有显著差异（p>0.05）。在实验2中，A组和B组的妊娠率/产仔率以及出生时的窝仔数/断奶时的窝仔数分别为86.7/86.7%、60.7/57.1%（p<0.05）和6.1±1.6/5.7±1.7、4.0±1.4/3.8±1.4（p<0.05）。本研究表明，使用牛用输精滴管和奥西里斯导管进行新鲜精液人工授精的结果是等效的。解冻后用前列腺液稀释的冻融犬精液经奥西里斯导管进行阴道输精的结果并不理想。当使用奥西里斯导管经阴道注入解冻后添加前列腺液的精液时，妊娠率、产仔率和窝仔数均降低。
