Péres Valéria Flores, Saffi Jenifer, Melecchi Maria Inês S, Abad Fernanda C, Martinez Migdalia M, Oliveira Eniz Conceição, Jacques Rosângela Assis, Caramão Elina B
Laboratório de Genética Toxicológica, ULBRA, Canoas, RS, Brazil; Universidad de La Habana, Cuba.
J Chromatogr A. 2006 Feb 10;1105(1-2):148-53. doi: 10.1016/j.chroma.2005.08.036. Epub 2005 Aug 31.
Piperaceae family is original from tropical regions and it shows more than 700 species around the world. Piper gaudichaudianum Kunth is the specie more abundant in Brazil, occurring from Northeast to South Brazil. In this paper, it was investigated the influence of some experimental parameters on the pressurized liquid extraction (PLE) of P. gaudichaudianum Kunth leaves, using petroleum ether as extractor solvent. The optimization of the main variables involved in the PLE process (extraction temperature and time) has been done by response surface methodology (RSM) using, as responses, the extraction yield and the chromatographic profile (GC/MS) of the extracts. The optimized procedure employed 3 g of ground leaves, 10 min of extraction and one cycle of extraction at 85 degrees C. The major compounds present in the petroleum ether extracts were: palmitic acid, stearic acid and nerolidol. The results presented in this work show the possibility of using a fast and easy process to recover compounds from P. gaudichaudianum Kunth.
胡椒科原产于热带地区，全球有700多种。高迪胡椒（Piper gaudichaudianum Kunth）是巴西分布最广的物种，从巴西东北部到南部均有生长。本文研究了一些实验参数对以石油醚为萃取溶剂的高迪胡椒叶加压液体萃取（PLE）的影响。采用响应面法（RSM）对PLE过程中的主要变量（萃取温度和时间）进行了优化，以提取物的萃取率和色谱图（GC/MS）作为响应指标。优化后的方法使用3克磨碎的叶子，在85℃下萃取10分钟，萃取一个循环。石油醚提取物中的主要化合物有：棕榈酸、硬脂酸和橙花叔醇。这项工作的结果表明，有可能采用一种快速简便的方法从高迪胡椒中提取化合物。
