Choi S H, Lee S W, Hong Y S, Jeun J M, Min B W
Korea University, Ansan Hospital, Ansan Gyeonggi-Do, South Korea.
Emerg Med J. 2006 Feb;23(2):119-22. doi: 10.1136/emj.2004.020651.
This study investigated the effect of hypertonic saline on the role of polymorphonuclear neutrophils (PMNs) in the inflammatory response and the effect of hypertonic saline infused at different phases in relation to an inflammatory stimulus.
PMNs were isolated from peripheral blood of healthy volunteers (Boyum's method) and cultured in three different media ([Na+] = 140 mmol/l, 180 mmol/l, and 200 mmol/l). PMNs were then stimulated with fMLP (N-formyl-methionyl-leucyl-phenylalanine) and H2O2 synthesis was quantified by flow cytometry at 5, 30, 60, 120, and 180 minutes. PMNs were treated with hypertonic saline before, simultaneously with, and after fMLP stimulation, and H2O2 synthesis quantified again.
H2O2 synthesis was two or three times higher in fMLP stimulated than in non-stimulated PMNs, and it reached maximum level at 120 minutes. In the absence of fMLP stimulation, there was no significant difference between control and hypertonic saline with regard to activity of H2O2 synthesis. In the presence of fMLP stimulation, H2O2 synthesis significantly decreased in PMNs treated with hypertonic saline. There was no significant difference between the two hypertonic saline solutions ([Na+] = 180 mmol/l and 200 mmol/l) with regard to H2O2 synthesis. However, H2O2 synthesis decreased in PMNs treated with hypertonic saline before and simultaneously with fMLP stimulation, but was not significantly decreased in the cells treated with hypertonic saline after fMLP stimulation.
Hypertonic saline appears to decrease H2O2 in stimulated neutrophils. This may be a further beneficial role of hypertonic saline when used clinically as an early resuscitation fluid.
本研究调查了高渗盐水对多形核中性粒细胞（PMNs）在炎症反应中作用的影响，以及在与炎症刺激相关的不同阶段输注高渗盐水的效果。
从健康志愿者外周血中分离出PMNs（采用博伊姆方法），并在三种不同培养基（[Na⁺]=140 mmol/L、180 mmol/L和200 mmol/L）中培养。然后用N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸（fMLP）刺激PMNs，并在5、30、60、120和180分钟时通过流式细胞术对过氧化氢（H₂O₂）合成进行定量。在fMLP刺激前、同时及之后用高渗盐水处理PMNs，并再次对H₂O₂合成进行定量。
fMLP刺激的PMNs中H₂O₂合成比未刺激的PMNs高两到三倍，且在120分钟时达到最高水平。在没有fMLP刺激的情况下，对照与高渗盐水在H₂O₂合成活性方面没有显著差异。在有fMLP刺激的情况下，用高渗盐水处理的PMNs中H₂O₂合成显著降低。两种高渗盐溶液（[Na⁺]=180 mmol/L和200 mmol/L）在H₂O₂合成方面没有显著差异。然而，在fMLP刺激前和同时用高渗盐水处理的PMNs中H₂O₂合成降低，但在fMLP刺激后用高渗盐水处理的细胞中H₂O₂合成没有显著降低。
高渗盐水似乎可降低受刺激中性粒细胞中的H₂O₂。这可能是高渗盐水在临床上用作早期复苏液时的又一有益作用。
