Nakayama H, Weser E
Gastroenterology. 1975 Mar;68(3):480-7.
After small bowel resection in the rat, mucosal hyperplasia and an increase in nucleic acid synthesis and cell proliferation occur in remaining small intestine. Male Sprague-Dawley rate underwent resection of 50 cm of proximal or distal intestine or sham operation. One month and 6 months after surgery, aspartate transcarbamylase, dihydroorotase, and uridine kinase were assayed in whole mucosa, and in some instances, in crypt mucosa ffrom the remaining intestinal segment. In control bowel, enzyme activity was significantly greater proximal compared with distal segments. One month after proximal or distal resection, mucosal enzyme activity per cm of gut was greater in the remnant bowel compared with controls. There was no such difference at 6 months. Specific enzyme activity of whole mucosa did not increase after resection because the assay was influenced by the disproportionately large contribution of villous protein. Specific enzyme activity (including thymidine kinase) of isolated crypt mucosa was significantly increased 1 month after distal resection. In addition, [3H]thymidine uptake into DNA of crypt mucosa from proximal remnants was also significantly increased. These results indicate that after small bowel resection, the enzymes of pryimidine biosynthesis increase in remaining bowel and parallel the accelerated rate of cell proliferation.
大鼠小肠切除术后，剩余小肠会出现黏膜增生以及核酸合成和细胞增殖增加的情况。雄性斯普拉格-道利大鼠接受了50厘米近端或远端小肠切除术或假手术。术后1个月和6个月，对整个黏膜以及某些情况下剩余肠段的隐窝黏膜中的天冬氨酸转氨甲酰酶、二氢乳清酸酶和尿苷激酶进行了测定。在对照肠段中，近端的酶活性明显高于远端。近端或远端切除术后1个月，与对照组相比，残余肠段每厘米肠道的黏膜酶活性更高。6个月时没有这种差异。由于测定受到绒毛蛋白不成比例的巨大贡献的影响，切除术后整个黏膜的比酶活性没有增加。远端切除术后1个月，分离的隐窝黏膜的比酶活性（包括胸苷激酶）显著增加。此外，来自近端残余物的隐窝黏膜中[3H]胸苷掺入DNA的量也显著增加。这些结果表明，小肠切除术后，嘧啶生物合成的酶在剩余肠段中增加，并与细胞增殖的加速速率平行。
