Salleh N, Baines D L, Naftalin R J, Milligan S R
Division of Reproductive Health, Endocrinology & Development, Guy's Campus, King's College, London, SE1 1UL, UK.
J Membr Biol. 2005 Jul;206(1):17-28. doi: 10.1007/s00232-005-0770-7.
The secretion of uterine luminal fluid initially provides a transport and support medium for spermatozoa and unimplanted embryos, while the absorption of uterine luminal fluid in early pregnancy results in the closure of the lumen and allows blastocysts to establish intimate contact with the uterine epithelium. We have established an in vivo perfusion technique of the lumen to study the hormonal control of the events in the peri-implantation period. Fluorescein-labelled dextran was included in the perfusion medium to monitor fluid movements and the concentrations of Na(+) and CI(-) ions in the effluent were monitored. Using an established regimen of steroid treatment of ovariectomized rats mimicking early pregnancy, oestradiol caused fluid secretion, while progesterone resulted in an amiloride-sensitive fluid absorption. Fluid absorption peaked at about the expected time of implantation. The effect of progesterone could be inhibited by treatment with a high dose of oestradiol, by the anti-progestin RU486, and by the presence of an intra-uterine contraceptive device. Studies of expression of Na(+) and CI(-) channels (ENaC, CFTR) indicated that these channels were subject to tissue-specific regulation within the uterus, but more work is required to determine their role and the factors controlling their abundance and localization in early pregnancy.
子宫腔液的分泌最初为精子和未着床胚胎提供运输和支持介质，而妊娠早期子宫腔液的吸收导致管腔闭合，并使囊胚与子宫上皮建立密切接触。我们建立了一种子宫腔体内灌注技术，以研究着床前期事件的激素调控。灌注介质中加入了荧光素标记的右旋糖酐以监测液体流动，并监测流出液中Na(+)和CI(-)离子的浓度。使用既定的模拟早期妊娠的卵巢切除大鼠类固醇治疗方案，雌二醇引起液体分泌，而孕酮导致氨氯地平敏感的液体吸收。液体吸收在预期的着床时间左右达到峰值。高剂量雌二醇治疗、抗孕激素RU486以及宫内节育器的存在可抑制孕酮的作用。对Na(+)和CI(-)通道（ENaC、CFTR）表达的研究表明，这些通道在子宫内受到组织特异性调控，但需要更多工作来确定它们在早期妊娠中的作用以及控制其丰度和定位的因素。
