Zhu C, Ruan L, Peng D, Yu Z, Sun M
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, China.
Lett Appl Microbiol. 2006 Feb;42(2):109-14. doi: 10.1111/j.1472-765X.2005.01817.x.
The objective of this work was to enhance the insecticidal activity or widen the pesticidal spectrum of a commercial Bacillus thuringiensis strain YBT1520.
A vegetative insecticidal protein gene vip3Aa7, under the control of its native promoter and cry3A promoter, was subcloned into B. thuringiensis acrystalliferous BMB171 to generate BMB8901 and BMBvip respectively. It was found that the amount of Vip3Aa7 protein produced by BMBvip was 3.2-fold more than that produced by BMB8901. Therefore, the vip3Aa7 gene under the control of cry3A promoter was transformed into strain YBT1520. The toxicity of the resulting strain BMB218V against Spodoptera exigua was 10-fold more than that of YBT1520, and that the toxicity of BMB218V against Helicoverpa armigera retained the same level as that of strain YBT1520.
Strain YBT1520 obtained high toxicity against S. exigua after it was transformed and expressed the foreign vip3Aa7 gene.
Commercial B. thuringiensis strain YBT1520 has high toxicity against H. armigera and Plutella xylostella, but almost no activity against S. exigua, which is a major crop pest in China. This work provides a new strategy for widening the activity spectrum of B. thuringiensis against agriculture pests.
本研究旨在增强商业苏云金芽孢杆菌菌株YBT1520的杀虫活性或拓宽其杀虫谱。
将营养期杀虫蛋白基因vip3Aa7在其天然启动子和cry3A启动子的控制下，亚克隆到无晶体苏云金芽孢杆菌BMB171中，分别构建BMB8901和BMBvip。发现BMBvip产生的Vip3Aa7蛋白量比BMB8901产生的多3.2倍。因此，将cry3A启动子控制下的vip3Aa7基因转化到菌株YBT1520中。所得菌株BMB218V对甜菜夜蛾的毒性比YBT1520高10倍，且BMB218V对棉铃虫的毒性与菌株YBT1520保持相同水平。
菌株YBT1520在转化并表达外源vip3Aa7基因后，对甜菜夜蛾具有高毒性。
商业苏云金芽孢杆菌菌株YBT1520对棉铃虫和小菜蛾具有高毒性，但对中国主要农作物害虫甜菜夜蛾几乎没有活性。本研究为拓宽苏云金芽孢杆菌对农业害虫的活性谱提供了新策略。
