Itil Ismail Mete, Cirpan Teksin, Akercan Fuat, Gamaa Akram, Kazandi Mert, Kazandi Ali Can, Yildiz Pinar Solmaz, Askar Niyazi
Department of Obstetrics and Gynecology, Ege University Faculty of Medicine, Izmir, Turkey.
Aust N Z J Obstet Gynaecol. 2006 Feb;46(1):38-41. doi: 10.1111/j.1479-828X.2006.00512.x.
To investigate the effect of Bacillus Calmette-Guerin (BCG) vaccine on peritoneal implantation of endometrial tissue in rats.
Forty sexually mature virgin Wistar albino rats weighing 190-200 g were randomly assigned (double blind) to two groups. The rats in the first group were vaccinated with 0.1 mL BCG and those in the second group were injected with 0.1 mL saline into the tail, intracutaneously. All the rats underwent median laparotomy after 4 weeks of vaccination or injection. The right uterine horn was excised, and the two samples of endometrial tissue dissected from myometrium were implanted on each side of peritoneum at the 2 cm lateral line of the median laparotomy incision. The implanted peritoneal segments were excised after 8 weeks of laparotomy. The tissue samples were accepted, histologically, as endometriosis when both glands and stroma of endometrial tissue were seen in sections.
Thirty-six implants from the study group and 34 implants from the control group were obtained. Ten and 23 implants were accepted as endometriosis in the study and control group, respectively. The number of endometriotic foci were significantly lower in the study group than in the control group (P = 0.01).
Stimulation of the cellular immune response with BCG vaccine could exert an inhibitory effect on ectopic endometriotic implants.
探讨卡介苗（BCG）对大鼠子宫内膜组织腹腔种植的影响。
将40只体重190 - 200 g的性成熟未孕Wistar白化大鼠随机（双盲）分为两组。第一组大鼠尾根部皮内注射0.1 mL卡介苗，第二组大鼠尾根部皮内注射0.1 mL生理盐水。接种疫苗或注射后4周，所有大鼠均行正中剖腹术。切除右侧子宫角，从子宫肌层分离出的两份子宫内膜组织样本分别植入正中剖腹术切口旁2 cm处的腹膜两侧。剖腹术后8周切除植入的腹膜节段。当组织切片中同时可见子宫内膜组织的腺体和间质时，组织学上将组织样本诊断为子宫内膜异位症。
研究组获得36个植入物，对照组获得34个植入物。研究组和对照组分别有10个和23个植入物被诊断为子宫内膜异位症。研究组的子宫内膜异位病灶数量明显低于对照组（P = 0.01）。
卡介苗疫苗刺激细胞免疫反应可对异位子宫内膜植入物产生抑制作用。
