Niku Mikael, Ekman Anna, Pessa-Morikawa Tiina, Iivanainen Antti
Department of Basic Veterinary Sciences, University of Helsinki, Helsinki, Finland.
BMC Vet Res. 2006 Jan 27;2:5. doi: 10.1186/1746-6148-2-5.
Identification of cell types in bovine tissue sections is complicated by the limited availability of anti-bovine antibodies, and by antigen retrieval treatments required for formalin-fixed tissue samples. We have evaluated an antibody and lectin panel for identifying major cell types in paraffin-embedded bovine tissue sections, and report optimized pretreatments for these markers.
We selected 31 useful antibodies and lectins which can be used to identify cell types of epithelia, connective tissue, muscle, and nervous tissue, as well as cell proliferation and apoptosis.
The panel of markers allows the identification of all major cell types in paraffin-embedded cattle tissue sections by immunohistochemistry or lectin histochemistry. Heat-induced epitope retrieval methods are required for most antibodies.
牛组织切片中细胞类型的鉴定因抗牛抗体的可用性有限以及福尔马林固定组织样本所需的抗原修复处理而变得复杂。我们评估了一组抗体和凝集素，用于鉴定石蜡包埋牛组织切片中的主要细胞类型，并报告了这些标志物的优化预处理方法。
我们选择了31种有用的抗体和凝集素，可用于鉴定上皮、结缔组织、肌肉和神经组织的细胞类型，以及细胞增殖和凋亡。
该标志物组可通过免疫组织化学或凝集素组织化学鉴定石蜡包埋牛组织切片中的所有主要细胞类型。大多数抗体需要热诱导抗原修复方法。
