[使用多位点序列分型和脉冲场凝胶电泳研究来自卡萨布兰卡（摩洛哥）的B群脑膜炎奈瑟菌分离株]

[Use of multilocus sequence typing and pulsed-field gel electrophoresis for the study of serogroup B Neisseria meningitidis isolates from Casablanca (Morocco)].

作者信息

Zerouali K, Castelli P, Van Looveren M, El Mdaghri N, Boudouma M, Benbachir M, Nicolas P

机构信息

Laboratoire de microbiologie, faculté de médecine de Casablanca,19, rue Tarik-Bnou-Zyad, BP 9154, 20000, Casablanca, Maroc.

出版信息

Pathol Biol (Paris). 2006 Apr;54(3):166-70. doi: 10.1016/j.patbio.2005.09.002. Epub 2006 Jan 25.

DOI:10.1016/j.patbio.2005.09.002

PMID:16442242

Abstract

A previous study showed that B:4:P1.15 was the most frequent phenotype of Neisseria meningitidis isolated in Casablanca (Morocco). To determine if there was an epidemic clone, MLST and PFGE were used to compare 13 B:4:P1.15 strains isolated from September 1999 to December 2000. MLST showed 4 Sequence Types (ST): ST-33 was the most frequent ST (9/13 strains) and 4 strains belonged to 3 newly described STs. Twelve stains belonged to ST-32 complex, and one strain presenting a new ST (ST-2502) did not belong to any known ST complex. The analysis by PFGE showed that the strains were subdivided into 7 clusters, and that there was no epidemic clone. MLST is useful for long-term epidemiological studies on N. meningitidis strains from varied geographical origins. PFGE seemed to be well adapted to the comparison of a small number of strains isolated during a short period within a defined community.

摘要

先前的一项研究表明，B:4:P1.15是在卡萨布兰卡（摩洛哥）分离出的脑膜炎奈瑟菌最常见的表型。为了确定是否存在流行克隆，采用多位点序列分型（MLST）和脉冲场凝胶电泳（PFGE）对1999年9月至2000年12月分离出的13株B:4:P1.15菌株进行比较。MLST显示有4种序列型（ST）：ST-33是最常见的ST（9/13株），4株属于3种新描述的ST。12株属于ST-32复合体，1株呈现新的ST（ST-2502），不属于任何已知的ST复合体。PFGE分析表明，这些菌株被细分为7个簇，且不存在流行克隆。MLST对于来自不同地理区域的脑膜炎奈瑟菌菌株的长期流行病学研究很有用。PFGE似乎非常适合比较在特定社区内短时间内分离出的少数菌株。