Gao Ying, Tian Yiling, Sun Xiuhua, Yin Xue-Bo, Xiang Qian, Ma Ge, Wang Erkang
Department of Chemistry, Tianjin University, Tianjin, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Mar 7;832(2):236-40. doi: 10.1016/j.jchromb.2006.01.004. Epub 2006 Jan 24.
The fast analysis of ranitidine is of clinical importance in understanding its efficiency and a patient's treatment history. In this paper, a novel determination method for ranitidine based on capillary electrophoresis-electrochemiluminescence detection is described. The conditions affecting separation and detection were investigated in detail. End-column detection of ranitidine in 5 mM Ru(bpy)(3)(2+) solution at applied voltage of 1.20 V was performed. Favorable ECL intensity with higher column efficiency was achieved by electrokinetic injection for 10s at 10 kV. The R.S.D. values of ECL intensity and migration time were 6.38 and 1.84% for 10(-4) M and 6.01 and 0.60% for 10(-5) M, respectively. A detection limit of 7 x 10(-8) M (S/N=3) was achieved. The proposed method was applied satisfactorily to the determination of ranitidine in urine in 6 min.
雷尼替丁的快速分析对于理解其疗效和患者治疗史具有临床重要性。本文描述了一种基于毛细管电泳 - 电化学发光检测的雷尼替丁新型测定方法。详细研究了影响分离和检测的条件。在1.20 V的施加电压下，于5 mM Ru(bpy)(3)(2+)溶液中对雷尼替丁进行柱端检测。通过在10 kV下电动进样10 s，实现了具有更高柱效的良好电化学发光强度。对于10(-4) M，电化学发光强度和迁移时间的相对标准偏差（R.S.D.）值分别为6.38%和1.84%；对于10(-5) M，分别为6.01%和0.60%。实现了7 x 10(-8) M（S/N = 3）的检测限。该方法在6分钟内令人满意地应用于尿液中雷尼替丁的测定。
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