Xu Yuanhong, Gao Ying, Wei Hui, Du Yan, Wang Erkang
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Graduate School of the Chinese Academy of Science, Changchun, Jilin 130022, China.
J Chromatogr A. 2006 May 19;1115(1-2):260-6. doi: 10.1016/j.chroma.2006.02.084. Epub 2006 Apr 17.
Capillary electrophoresis (CE) with Ru(bpy)3(2+) electrochemiluminescence (ECL) detection system was established to the determination of contamination of banknotes with controlled drugs and a high efficiency on-column field-amplified sample stacking (FASS) technique was also optimized to increase the ECL intensity. The method was illustrated using heroin and cocaine, which are two typical and popular illicit drugs. Highest sample stacking was obtained when 0.01 mM acetic acid was chosen for sample dissolution with electrokinetical injection for 6 s at 17 kV. Under the optimized conditions: ECL detection at 1.2 V, separation voltage 10.0 kV, 20 mM phosphate-acetate (pH 7.2) as running buffer, 5 mM Ru(bpy)3(2+) with 50 mM phosphate-acetate (pH 7.2) in the detection cell, the standard curves were linear in the range of 7.50x10(-8) to 1.00x10(-5) M for heroin and 2.50x10(-7) to 1.00x10(-4) M for cocaine and detection limits of 50 nM for heroin and 60 nM for cocaine were achieved (S/N = 3), respectively. Relative standard derivations of the ECL intensity and the migration time were 3.50 and 0.51% for heroin and 4.44 and 0.12% for cocaine, respectively. The developed method was successfully applied to the determination of heroin and cocaine on illicit drug contaminated banknotes without any damage of the paper currency. A baseline resolution for heroin and cocaine was achieved within 6 min.
建立了基于钌（联吡啶）3（2+）电化学发光（ECL）检测系统的毛细管电泳（CE）方法，用于测定纸币上的管制药物污染情况，同时优化了高效的柱上场放大进样（FASS）技术以增强ECL强度。该方法以海洛因和可卡因这两种典型且常见的非法药物为例进行了说明。当选择0.01 mM乙酸溶解样品并在17 kV下电动进样6 s时，可实现最高的样品堆积。在优化条件下：检测电压为1.2 V，分离电压为10.0 kV，运行缓冲液为20 mM磷酸盐 - 乙酸盐（pH 7.2），检测池中含有5 mM钌（联吡啶）3（2+）和50 mM磷酸盐 - 乙酸盐（pH 7.2），海洛因的标准曲线在7.50×10（-8）至1.00×10（-5）M范围内呈线性，可卡因的标准曲线在2.50×10（-7）至1.00×10（-4）M范围内呈线性，海洛因和可卡因的检测限分别为50 nM和60 nM（S/N = 3）。海洛因的ECL强度和迁移时间的相对标准偏差分别为3.50%和0.51%，可卡因为4.44%和0.12%。所建立的方法成功应用于测定被非法药物污染的纸币上的海洛因和可卡因，且不会对纸币造成任何损坏。在6分钟内实现了海洛因和可卡因的基线分离。
