Isolation and characterization of new restriction endonucleases from Vibrio parahaemolyticus: VpaK32I enzyme with the class-IIS heptanucleotide specificity, GCTCTTCN1/N4.
作者信息
Miyahara M, Shimada T, Kotani H, Mise K
机构信息
Division of Microbiology, National Institute of Hygienic Sciences, Tokyo, Japan.
出版信息
Gene. 1992 Aug 1;117(1):103-6. doi: 10.1016/0378-1119(92)90496-c.
PMID:1644299
Abstract
Six restriction endonucleases (ENases), classified into four different specificities, were found in a screen among 68 reference strains of Vibrio parahaemolyticus of human origin. Five of these ENases are isoschizomers of well-known ENases, while the remaining one, designated VpaK32I, is a novel and highly efficient class-IIS ENase with the hepatanucleotide recognition site, 5'-GCTCTTC(1/4)-3'.
摘要
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