Cis-acting control elements 5' to aldA, the aldehyde dehydrogenase-encoding gene of Aspergillus niger.
作者信息
O'Connell M J, Kelly J M
机构信息
Department of Genetics, University of Adelaide, S.A., Australia.
出版信息
Gene. 1992 Aug 1;117(1):151-6. doi: 10.1016/0378-1119(92)90505-j.
PMID:1644309
Abstract
The aldA gene of Aspergillus niger encodes an aldehyde dehydrogenase. Chromosomal deletion of this gene using gene replacement techniques had defined several growth conditions, thought to involve aldehyde and semialdehyde intermediate, that require this enzyme. Reintroduction of aldA clones carrying in vitro-generated deletions in the promoter region into the aldA deletion strain has been used to identify three elements controlling aldA expression: an enhancer required for high-level expression, an ethanol induction-responsive element, and a fructose induction-responsive element.
摘要
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