Gaucher Sara P, Hadi Masood Z, Young Malin M
Sandia National Laboratories, Livermore, California 94551-0969, USA.
J Am Soc Mass Spectrom. 2006 Mar;17(3):395-405. doi: 10.1016/j.jasms.2005.11.023. Epub 2006 Jan 27.
A systematic study of the dissociation patterns of crosslinked peptides analyzed by tandem mass spectrometry is reported. A series of 11-mer peptides was designed around either a polyalanine or polyglycine scaffold with arginine at the C terminus. One or two lysine residues were included at various locations within the peptides to effect inter- or intra-molecular crosslinking, respectively. Crosslinked species were generated with four commonly used amine-specific chemical crosslinking reagents: disuccinimidyl suberate (DSS), disuccinimidyl tartarate (DST), dithiobis(succinimidylpropionate) (DSP), and disuccinimidyl glutarate (DSG). The influence of precursor charge state, location of crosslink, and specific crosslinking reagent on the MS/MS dissociation pattern was examined. Observed trends in the dissociation patterns obtained for these species will allow for improvements to software used in the automated interpretation of crosslinked peptide MS/MS data.
本文报道了一项通过串联质谱分析交联肽解离模式的系统研究。围绕聚丙氨酸或聚甘氨酸支架设计了一系列11聚体肽，其C端为精氨酸。在肽内的不同位置分别包含一个或两个赖氨酸残基，以分别实现分子间或分子内交联。使用四种常用的胺特异性化学交联试剂生成交联物种：辛二酸二琥珀酰亚胺酯（DSS）、酒石酸二琥珀酰亚胺酯（DST）、二硫代双（琥珀酰亚胺丙酸酯）（DSP）和戊二酸二琥珀酰亚胺酯（DSG）。研究了前体电荷状态、交联位置和特定交联试剂对MS/MS解离模式的影响。观察到的这些物种解离模式的趋势将有助于改进用于自动解释交联肽MS/MS数据的软件。
