Villarreal Daniel, Reams Garry, Freeman Ronald, Spear Robert, Tchoukina Inna, Samar Huma
Department of Internal Medicine, SUNY Upstate Medical University, Syracuse, NY 13210, USA.
Mol Cell Biochem. 2006 Feb;283(1-2):153-7. doi: 10.1007/s11010-006-2567-0.
Previous investigations in normotensive animals have demonstrated a marked natriuretic and diuretic response following the acute administration of supraphysiologic doses of synthetic leptin. However, the importance of endogenous leptin in the regulation of renal sodium and water balance is not yet defined. This study examined the hemodynamic and renal excretory effects of circulating leptin blockade with a specific polyclonal antibody in groups of normotensive, chronically saline-loaded Sprague-Dawley rats. In the experimental group (n = 10), leptin antibody significantly decreased urinary sodium excretion and urinary flow by approximately 30% compared to the control rats (n = 10). Mean arterial pressure remained unchanged. Collectively, these results are interpreted to suggest that leptin is an important renal sodium-regulating factor under conditions of mild sodium and volume expansion.
先前对正常血压动物的研究表明，急性给予超生理剂量的合成瘦素后会出现明显的利钠和利尿反应。然而，内源性瘦素在调节肾钠和水平衡中的重要性尚未明确。本研究在正常血压、长期生理盐水负荷的Sprague-Dawley大鼠组中，用特异性多克隆抗体检测了循环瘦素阻断对血流动力学和肾脏排泄的影响。在实验组（n = 10）中，与对照大鼠（n = 10）相比，瘦素抗体使尿钠排泄和尿流量显著降低了约30%。平均动脉压保持不变。总体而言，这些结果被解释为表明在轻度钠和容量扩张的情况下，瘦素是一种重要的肾钠调节因子。
