关于SFPR全细胞生物催化拜耳-维利格氧化过程中氧气和细胞浓度的影响

On the influence of oxygen and cell concentration in an SFPR whole cell biocatalytic Baeyer-Villiger oxidation process.

作者信息

Hilker Iris, Baldwin Chris, Alphand Véronique, Furstoss Roland, Woodley John, Wohlgemuth Roland

机构信息

Department of Biochemistry, Fluka Group, Industriestrasse 25, CH-9470 Buchs, Switzerland.

出版信息

Biotechnol Bioeng. 2006 Apr 20;93(6):1138-44. doi: 10.1002/bit.20829.

DOI:10.1002/bit.20829

PMID:16444739

Abstract

Efficient whole cell biotransformations, in particular microbial whole cell Baeyer-Villiger oxidation with molecular oxygen, demand comprehension and optimization of the process details involved. Optimal provision of oxygen and control of bioprocess parameters are pivotal for their success. The interrelation of cell density and oxygen supply in an in situ substrate feeding and product removal (SFPR) whole cell Baeyer-Villiger oxidation process was investigated in detail. Both parameters were optimized with respect to practical considerations. The outcome of this study supports a schematic process model, allows estimation of optimum process conditions and exploration of its limits.

摘要

高效的全细胞生物转化，特别是利用分子氧进行的微生物全细胞拜耳-维利格氧化反应，需要对所涉及的工艺细节进行理解和优化。氧气的最佳供应和生物工艺参数的控制对其成功至关重要。详细研究了原位底物进料和产物去除（SFPR）全细胞拜耳-维利格氧化过程中细胞密度与氧气供应的相互关系。从实际考虑出发，对这两个参数进行了优化。本研究结果支持一个示意性的工艺模型，能够估计最佳工艺条件并探索其极限。