Kayaselcuk F, Erkanli S, Bolat F, Seydaoglu G, Kuscu E, Demirhan B
Department of Pathology, Adana Research and Medical Center, Baskent University, Yuregir 01350, Adana, Turkey.
Int J Gynecol Cancer. 2006 Jan-Feb;16(1):402-8. doi: 10.1111/j.1525-1438.2006.00407.x.
Cyclins are known as regulatory proteins in cell cycle. Cyclin H is a part of cyclin H/Cdk7/Mat1 complex, which is necessary for cellular proliferation. This study was designed to investigate the correlation of cyclin H expression with tumorigenesis of the endometrium and clinicopathologic variables. Immunohistochemical staining using labeled streptavidin-biotin complex was performed on formalin-fixed, paraffin-embedded endometrial tissues of the proliferative, hyperplastic, and carcinomatous types. Immunostaining for cyclins A, B1, D1, D3, E, H, and cyclin dependent kinase 2 were evaluated. The expression of cyclins A, D1, D3, and H in hyperplasia was significantly more frequent than those of proliferative phase and less than those of endometrioid adenocarcinoma. The expression of cyclin H was correlated with lymphvascular space invasion and clinical stage in carcinoma but not with myometrial invasion, lymph node metastasis, and menopause status. The expression of cyclin H could be involved in the transformation of the endometrium into malignancy and might be a marker for more proliferative and malignant features. It might be one of the biomarkers for determining proliferative activity in endometrial hyperplasia and endometrioid adenocarcinoma.
细胞周期蛋白是细胞周期中的调控蛋白。细胞周期蛋白H是细胞周期蛋白H/Cdk7/Mat1复合物的一部分，对细胞增殖至关重要。本研究旨在探讨细胞周期蛋白H表达与子宫内膜肿瘤发生及临床病理变量之间的相关性。对增殖期、增生期和癌性福尔马林固定、石蜡包埋的子宫内膜组织进行了使用标记链霉亲和素-生物素复合物的免疫组织化学染色。评估了细胞周期蛋白A、B1、D1、D3、E、H以及细胞周期蛋白依赖性激酶2的免疫染色情况。细胞周期蛋白A、D1、D3和H在增生期的表达明显比增殖期更频繁，且低于子宫内膜样腺癌。细胞周期蛋白H的表达与癌中的淋巴管间隙浸润和临床分期相关，但与肌层浸润、淋巴结转移和绝经状态无关。细胞周期蛋白H的表达可能参与子宫内膜向恶性的转变，并且可能是更具增殖性和恶性特征的标志物。它可能是确定子宫内膜增生和子宫内膜样腺癌增殖活性的生物标志物之一。
