大鼠血浆中反式白藜芦醇的高效液相色谱光谱分析
High-performance liquid chromatography spectrometric analysis of trans-resveratrol in rat plasma.
作者信息
He Hui, Chen Xijing, Wang Guangji, Wang Jiping, Davey Andrew Keith
机构信息
Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing, Jiangsu 210009, China.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Mar 7;832(2):177-80. doi: 10.1016/j.jchromb.2005.12.021. Epub 2006 Jan 30.
A HPLC method for determination of trans-resveratrol concentrations in rat plasma was developed. Plasma samples were treated with acetonitrile to deposit proteins. The analysis used a Hypersil ODS(2) C(18) column (5 microm, 4.6 mm x 250 mm) and methanol/distilled water as the mobile phase (flow-rate=1 mL/min). The UV detection wavelength was 303 nm, and chlorzoxazone was used as the internal standard. The calibration curve was linear over the range of 0.02-40 microg/mL with a correlation coefficient of 0.9997. This concentration range corresponds well with the plasma concentrations of resveratrol in pharmacokinetic studies. There was 98.7%, 91.3% and 84.4% recovery from 0.02, 0.4 and 40 microg/mL plasma samples respectively. The R.S.D. of intra- and inter-day assay variations were all less than 12%. This HPLC assay is a quick, precise and reliable method for the analysis of resveratrol in pharmacokinetic studies.
建立了一种测定大鼠血浆中反式白藜芦醇浓度的高效液相色谱法。血浆样品用乙腈处理以沉淀蛋白质。分析采用Hypersil ODS(2) C(18) 柱（5微米，4.6毫米×250毫米），以甲醇/蒸馏水为流动相（流速=1毫升/分钟）。紫外检测波长为303纳米，氯唑沙宗用作内标。校准曲线在0.02 - 40微克/毫升范围内呈线性，相关系数为0.9997。该浓度范围与药代动力学研究中白藜芦醇的血浆浓度非常吻合。从0.02、0.4和40微克/毫升血浆样品中的回收率分别为98.7%、91.3%和84.4%。日内和日间测定变异的相对标准偏差均小于12%。这种高效液相色谱法是药代动力学研究中分析白藜芦醇的一种快速、精确且可靠的方法。
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