免疫组织化学作为一种研究人声带弹性纤维的方法。
Immunohistochemistry as a method to study elastic fibers of human vocal fold.
作者信息
Ramos Hugo Valter Lisboa, Simões Manuel de Jesus, Pontes Paulo Augusto de Lima, Neves Luciano Rodrigues, Barbosa Luiz Henrique Fonceca, De Biase Noemi Grigoletto, Oshima Celina T S
机构信息
UNIFESP, Escola Paulista de Medicina.
出版信息
Braz J Otorhinolaryngol. 2005 Jul-Aug;71(4):486-91. doi: 10.1016/s1808-8694(15)31204-0. Epub 2005 Dec 15.
AIM
Verify the use of immunohistochemistry as a method to measure all forms of elastic fibers at human vocal folds.
STUDY DESIGN
Transversal cohort.
MATERIAL AND METHOD
We collected vocal folds following these criterion: age between 25 and 40, Caucasian men, dead by gun shot, within 12 hours of death, without instrumentation of the larynx or suspicion of neck injury and without mucosal lesions noted by microscopy. Ten vocal folds were collected and one, of a man aged 28 years, was selected to study. The vocal fold was transversely cut in 9 regions and in each segment three slides were made. These slides were stained by Verhoeff and Weighert's resorcin-fuchsin and used for immunohistochemistry. The elastic compound was measured by colorimetric software analysis.
RESULTS
In Verhoeff and Weighert's resorcin-fuchsin, the intermediate and deep layer showed values higher than those of the superficial layer. The amount of tropoelastin identified by the antibody at the superficial layer was close to those of intermediate and deep layer.
CONCLUSION
Immunohistochemistry is a method that can identify and measure all forms of elastic fibers at human vocal fold.
目的
验证免疫组织化学作为一种测量人声带所有形式弹性纤维的方法的实用性。
研究设计
横向队列研究。
材料与方法
我们按照以下标准收集声带：年龄在25至40岁之间的白种男性，死于枪击，死亡时间在12小时内，无喉部器械操作或颈部损伤嫌疑，且显微镜检查未发现黏膜病变。收集了10条声带，选择其中一名28岁男性的声带进行研究。将声带横向切成9个区域，每个切片制作3张玻片。这些玻片用Verhoeff和Weighert间苯二酚品红染色，并用于免疫组织化学。通过比色软件分析测量弹性复合物。
结果
在Verhoeff和Weighert间苯二酚品红染色中，中层和深层的值高于表层。抗体在表层识别的原弹性蛋白量与中层和深层接近。
结论
免疫组织化学是一种能够识别和测量人声带所有形式弹性纤维的方法。
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