Harada F, Sawyer R C, Dahlberg J E
J Biol Chem. 1975 May 10;250(9):3487-97.
The nucleotide sequence of an RNA primer molecule for initiation of Rous sarcoma virus DNA synthesis in vitro has been determined. The sequence can be drawn in a cloverleaf structure typical of tRNAs with an anticodon for tryptophan. Aminoacylation of the molecule confirms that it is tRNA-Trp. The same sequence and aminoacylation results are obtained regardless of whether the RNA is isolated from virions or from cells of chickens, the natural host for this virus. It is the only species of tRNA-Trp that is dectected in chicked cell tRNA.
已确定了用于体外启动劳氏肉瘤病毒DNA合成的RNA引物分子的核苷酸序列。该序列可以绘制成具有色氨酸反密码子的典型tRNA的三叶草结构。该分子的氨酰化作用证实它是tRNA-Trp。无论RNA是从病毒粒子中分离出来的，还是从该病毒的天然宿主鸡的细胞中分离出来的，都能得到相同的序列和氨酰化结果。它是在鸡细胞tRNA中检测到的唯一一种tRNA-Trp。
