Przerwa Anna, Zimecki Michał, Switała-Jeleń Kinga, Dabrowska Krystyna, Krawczyk Ewa, Łuczak Mirosław, Weber-Dabrowska Beata, Syper Danuta, Miedzybrodzki Ryszard, Górski Andrzej
Transplantation Institute, Medical University of Warsaw, 02-006 Warsaw, Poland.
Med Microbiol Immunol. 2006 Sep;195(3):143-50. doi: 10.1007/s00430-006-0011-4. Epub 2006 Jan 31.
Reactive oxygen species (ROS) play a major role in mediating antibacterial functions of phagocytic cells. However, excessive ROS production may cause oxidative stress and tissue damage. Uncompensated ROS release has been implicated in a variety of disorders. Novel means of controlling elevated ROS production are urgently needed. We showed that homologous but not the heterologous phages inhibited, in a dose dependent manner, the degree of chemiluminescence in phagocytes induced by Escherichia coli. Treatment of the cells with the phages alone resulted in a small increase in ROS production. Homologous phages also facilitated phagocytosis when preincubated with bacteria. On the other hand, both homologous and heterologous phages inhibited phagocytosis following preincubation with phagocytic cells. The treatment of infected and uninfected mice with phages did not significantly alter the rate of phagocytosis by blood granulocytes and monocytes. In conclusion, we showed that bacteriophages can decrease ROS production by phagocytes. Although in some in vitro experimental models the phages tended to diminish phagocytosis, this phenomenon may be of little significance in clinical situations, since the process of eliminating bacteria in phage-treated patients is predominantly accomplished by both phages and phagocytes.
活性氧（ROS）在介导吞噬细胞的抗菌功能中起主要作用。然而，过量的ROS产生可能导致氧化应激和组织损伤。未得到补偿的ROS释放与多种疾病有关。迫切需要控制ROS产生增加的新方法。我们发现同源噬菌体而非异源噬菌体以剂量依赖的方式抑制大肠杆菌诱导的吞噬细胞中的化学发光程度。单独用噬菌体处理细胞会导致ROS产生略有增加。同源噬菌体与细菌预孵育时也促进吞噬作用。另一方面，同源和异源噬菌体在与吞噬细胞预孵育后均抑制吞噬作用。用噬菌体处理感染和未感染的小鼠并未显著改变血液粒细胞和单核细胞的吞噬速率。总之，我们发现噬菌体可降低吞噬细胞产生的ROS。虽然在一些体外实验模型中噬菌体倾向于减少吞噬作用，但这种现象在临床情况下可能意义不大，因为在噬菌体治疗的患者中消除细菌的过程主要由噬菌体和吞噬细胞共同完成。
