Ijichi Hideki, Taketomi Akinobu, Soejima Yuji, Yoshizumi Tomoharu, Uchiyama Hideaki, Shimada Mitsuo, Maehara Yoshihiko
The Department of Surgery, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.
Liver Int. 2006 Mar;26(2):248-53. doi: 10.1111/j.1478-3231.2005.01218.x.
The depletion of biochemical energy stores during prolonged cold storage is one of the most critical events of cold ischemia-reperfusion (CI/R) injury. The aim of this study was to evaluate the effect of hyperbaric oxygen (HBO) treatment on CI/R injury.
Livers were harvested from male Wistar rats and stored for 24 h at 4 degrees C in University of Wisconsin solution (Group 1). Others were additionally treated with HBO during the preservation period (Group 2). At the end of the 24 h cold preservation, the concentrations of hepatic enzymes and lipid peroxidation (LPO) in the effluent and the hepatic adenosine triphosphate (ATP) levels were measured. After preservation, the livers were reperfused for 90 min with an oxygenated Krebs-Henseleit bicarbonate buffer. Perfusate samples were obtained serially, and portal flow rates were also recorded.
In group 2, aspartate aminotransferase (AST), lactate dehydrogenase (LDH), and LPO into the effluent at the end of preservation were decreased and the depletion of ATP was prevented (P<0.05). After reperfusion, the portal flow was significantly improved in group 2 (P<0.05). The time-dependent increase of alanine aminotransferase levels (ALT) observed in group 1 was suppressed significantly in group 2, and total bile production during 90 min of reperfusion was significantly greater in group 2 (P<0.05). The structure of the livers in group 2 was significantly well maintained, and the liver weight change ratio was significantly greater in group 1 (P<0.05).
HBO treatment during cold storage seems to prevent hepatic ischemic injury and have protective effects against CI/R injury by attenuating the depletion of energy stores.
长时间冷藏期间生化能量储备的耗竭是冷缺血再灌注（CI/R）损伤最关键的事件之一。本研究的目的是评估高压氧（HBO）治疗对CI/R损伤的影响。
从雄性Wistar大鼠获取肝脏，并在4℃下于威斯康星大学溶液中保存24小时（第1组）。其他肝脏在保存期间额外接受HBO治疗（第2组）。在24小时冷保存结束时，测量流出液中肝酶浓度、脂质过氧化（LPO）以及肝脏三磷酸腺苷（ATP）水平。保存后，肝脏用充氧的 Krebs-Henseleit 碳酸氢盐缓冲液再灌注90分钟。连续获取灌注液样本，并记录门静脉流速。
在第2组中，保存结束时流出液中的天冬氨酸转氨酶（AST）、乳酸脱氢酶（LDH）和LPO降低，并且ATP的耗竭得到预防（P<0.05）。再灌注后，第2组的门静脉血流显著改善（P<0.05）。第1组中观察到的丙氨酸转氨酶水平（ALT）随时间的增加在第2组中被显著抑制，并且第2组在90分钟再灌注期间的总胆汁生成显著更多（P<0.05）。第2组肝脏的结构显著得到更好的维持，并且第1组的肝脏重量变化率显著更大（P<0.05）。
冷藏期间的HBO治疗似乎可预防肝脏缺血损伤，并通过减轻能量储备的耗竭对CI/R损伤具有保护作用。
