Briggs J A, Burrus G R, Stickney B D, Briggs R C
Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
J Cell Biochem. 1992 May;49(1):82-92. doi: 10.1002/jcb.240490114.
The human myeloid cell nuclear differentiation antigen (MNDA) is a protein of 406 amino acids that is expressed specifically in granulocytes, monocytes and earlier stage cells of these lineages. Degenerate oligonucleotides that could encode regions of MNDA amino acid sequence were used to amplify the MNDA cDNA sequence using the polymerase chain reaction. The amplified cDNA product was sequenced to confirm that it encoded the MNDA protein. It was then used as a probe to isolate five clones from a human bone marrow lambda gt10 cDNA library. A clone containing a 1,672 base pair cDNA insert was sequenced and found to encode the entire MNDA open reading frame, as well as 5' and 3' untranslated regions. The primary structure of the MNDA contains extensive regions of sequence similarity with the protein products of the interferon-inducible genes: 204 and interferon regulatory factor 2. In addition, a 12-base sequence matching the interferon-stimulated response element consensus sequence [GAAAN(N)GAAA] is located in the 5' untranslated region of the MNDA cDNA. The 1.8 kb MNDA mRNA was detected only in cells that express the antigen and the level of MNDA mRNA was elevated in cells treated with either recombinant or natural interferon alpha. The MNDA mRNA was not induced by interferon alpha in cells that do not exhibit a constitutive level of the MNDA mRNA. The MNDA contains sequence motifs found in gene regulatory proteins. The expression and the primary structure of the MNDA indicates that it plays a role in the granulocyte/monocyte cell-specific response to interferon.
人类髓样细胞核分化抗原（MNDA）是一种由406个氨基酸组成的蛋白质，在粒细胞、单核细胞以及这些谱系的早期阶段细胞中特异性表达。利用能够编码MNDA氨基酸序列区域的简并寡核苷酸，通过聚合酶链反应扩增MNDA cDNA序列。对扩增得到的cDNA产物进行测序，以确认其编码MNDA蛋白。然后将其用作探针，从人骨髓λgt10 cDNA文库中分离出5个克隆。对一个包含1672个碱基对cDNA插入片段的克隆进行测序，发现它编码整个MNDA开放阅读框以及5'和3'非翻译区。MNDA的一级结构包含与干扰素诱导基因的蛋白质产物：204和干扰素调节因子2具有广泛序列相似性的区域。此外，在MNDA cDNA的5'非翻译区存在一个与干扰素刺激反应元件共有序列[GAAAN(N)GAAA]匹配的12碱基序列。仅在表达该抗原的细胞中检测到1.8 kb的MNDA mRNA，在用重组或天然干扰素α处理的细胞中，MNDA mRNA水平升高。在不呈现MNDA mRNA组成型水平的细胞中，干扰素α不会诱导MNDA mRNA。MNDA包含在基因调节蛋白中发现的序列基序。MNDA的表达和一级结构表明它在粒细胞/单核细胞对干扰素的细胞特异性反应中起作用。
