Canaria Christie A, So Jonathan, Maloney James R, Yu C J, Smith Jeffrey O, Roukes Michael L, Fraser Scott E, Lansford Rusty
Department of Chemistry, California Institute of Technology, 1200 E. California Blvd., Pasadena, CA 91125, USA.
Lab Chip. 2006 Feb;6(2):289-95. doi: 10.1039/b510661c. Epub 2006 Jan 3.
We report the development of novel reagents and approaches for generating recyclable biosensors. The use of aqueous media for the formation of protein binding alkylthiolate monolayers on Au surfaces results in accelerated alkylthiolate monolayer formation and improvement in monolayer integrity as visualized by fluorescence microscopy and CV techniques. We have also developed an electrocleaning protocol that is compatible with microfluidics devices, and this technique serves as an on-chip method for cleaning Au substrates both before and after monolayer formation. The techniques for the formation and dissociation of biotinylated SAMs from aqueous solvents reported here may be applied towards the development of Au-based sensor devices and microfluidics chips in the future. A potential use of these devices includes the specific capture and triggered release of target cells, proteins, or small molecules from liquid samples.
