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巴西固氮螺菌Sp7中一个铁调节蛋白(Fur)同源物的克隆与特性分析

Cloning and characterization of a fur homologue from Azospirillum brasilense Sp7.

作者信息

Alahari Anuradha, Tripathi Anil Kumar, Le Rudulier Daniel

机构信息

Unité Interactions Plantes-Microorganismes et Santé végétale, CNRS-INRA-Université de Nice-Sophia Antipolis, France.

出版信息

Curr Microbiol. 2006 Feb;52(2):123-7. doi: 10.1007/s00284-005-0204-x. Epub 2006 Jan 31.

Abstract

A homologue of the ferric uptake regulator gene, fur, was identified from a Azospirillum brasilense Sp7 genomic DNA clone. Experiments performed with transcriptional lacZ fusions demonstrated that the A. brasilense fur homologue regulated the expression of two fur regulated Escherichia coli genes: fiu (ferric iron uptake) and fhuF (ferric hydroxamate uptake). A differential regulation by the cognate Fur and the heterologous Fur homologue in response to the iron status of the growth medium was also observed.

摘要

从巴西固氮螺菌Sp7基因组DNA克隆中鉴定出铁摄取调节基因fur的一个同源物。用转录lacZ融合体进行的实验表明,巴西固氮螺菌fur同源物调节两个受fur调节的大肠杆菌基因的表达:fiu(铁摄取)和fhuF(高铁羟肟酸摄取)。还观察到同源Fur和异源Fur同源物对生长培养基中铁状态的差异调节。

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