Cerny J, Waner E B
J Immunol. 1975 Feb;114(2 Pt 1):571-80.
Mice infected with murine oncogenic RNA viruses, either Friend virus (FV) or Moloney leukemia virus (MuLV-M) reacted well to a primary immunization with antigen(s) of Vibrio cholerae; both the kinetics and magnitude of the direct (IgM) primary vibriolytic antibody plaque forming cell (PFC) response in the spleen was the same as in non-infected control mice. In contrast, the anamnestic PFC (IgM) response of mice preimmunized with cholera and later (2 to 9 months following priming) infected with either FV or MuLV-M, before the secondary challenge, was inhibited 90% or more when compared to the anamnestic response of controls. Hemolytic PFC response to a single (primary) immunization with sheep red blood cells (sRBC, an antigen to which laboratory mice have a natural immune background) was suppressed by infection with both viruses in a manner similar to the suppression of the secondary vibiolytic response. The suppressive effect of FV, and the lack of it, on these different immune responses was correlated with the appearance of FV-induced cell membrane antigen, FVMA, on respective individual PFC from the spleen. FVMA was detected by inhibition of PFC in the presence of specific antiserum, anti-FVMA, and complement (C). In particular, about 80% of the FV-infected mice with an anamnestic response to a secondary challenge with cholera antigen had a significant decline in the proportion of PFC (33% or more) following incubation with anti-FVMA plus C. A similar degree of inhibition was found with hemolytic (anti-sRBC) PFC from FV-infected mice. In contrast, primary vibriolytic PFC from FV-infected mice were not inhibited by anti-FVMA, i.e., they did not carry the virus-induced antigen. These results indicate absolute correlation between viral suppression of a given clone of immunocompetent cells, and antigenic alteration of individual antibody-forming cells due to the virus-infection. Furthermore, the absence of both functional inhibition and virus-induced antigen(s) in the primary immunocompetent cells and the presence of both these virus-related functions in the memory cells (either experimentally sensitized cholera memory cells or naturally sensitized sRBC memory cells) strongly suggested that the latter are selectively susceptible to oncornavirus.
感染鼠致癌RNA病毒（即Friend病毒（FV）或莫洛尼白血病病毒（MuLV - M））的小鼠，对霍乱弧菌抗原的初次免疫反应良好；脾脏中直接（IgM）初次溶菌抗体空斑形成细胞（PFC）反应的动力学和强度与未感染的对照小鼠相同。相比之下，预先用霍乱免疫、随后（初次免疫后2至9个月）在二次攻击前感染FV或MuLV - M的小鼠，其回忆性PFC（IgM）反应与对照小鼠的回忆性反应相比，受到了90%或更多的抑制。用绵羊红细胞（sRBC，实验室小鼠对其有天然免疫背景的一种抗原）进行单次（初次）免疫后的溶血PFC反应，在两种病毒感染下均受到抑制，其方式类似于二次溶菌反应的抑制。FV对这些不同免疫反应的抑制作用及其缺乏抑制作用，与FV诱导的细胞膜抗原FVMA在脾脏中各个单独的PFC上的出现相关。通过在特异性抗血清抗FVMA和补体（C）存在下抑制PFC来检测FVMA。特别地，约80%对霍乱抗原二次攻击有回忆性反应的FV感染小鼠，在用抗FVMA加C孵育后，PFC比例显著下降（33%或更多）。在FV感染小鼠的溶血（抗sRBC）PFC中也发现了类似程度的抑制。相反，FV感染小鼠的初次溶菌PFC未被抗FVMA抑制，即它们不携带病毒诱导的抗原。这些结果表明，免疫活性细胞特定克隆的病毒抑制与病毒感染导致的单个抗体形成细胞的抗原改变之间存在绝对相关性。此外，初次免疫活性细胞中缺乏功能抑制和病毒诱导的抗原，而在记忆细胞（无论是实验致敏的霍乱记忆细胞还是天然致敏的sRBC记忆细胞）中存在这两种与病毒相关的功能，强烈表明后者对肿瘤病毒具有选择性易感性。
