Ruddle N H, Armstrong M K, Richards F F
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3714-8. doi: 10.1073/pnas.73.10.3714.
Murine lymphoid cells were infected in vitro with WN 1802 B, a naturally occurring murine leukemia virus isolated from the spleen of an 18-month-old BALB/c mouse. Normal spleen and bone marrow cells were more susceptible to infection than were cells prepared from thymus and lymph node. Spleen cells from athymic nu/nu mice also could be readily infected with virus. Permissive cells did not ingest iron readily infected with virus. Permissive cells did not ingest iron filings and did not adhere to plastic. Exogenous replication of murine leukemia virus was enhanced in spleen and lymph node cells treated with lipopolysaccharide, a bone marrow-derived lymphocyte mitogen. Conversely, cells treated with the thymus-derived lymphocyte cell mitogens, phytohemagglutinin and concanavalin A, were less capable of supporting murine leukemia virus replication. These studies suggest that the natural host for WN 1802 B is the bone marrow-derived lymphocyte.
用WN 1802 B体外感染小鼠淋巴细胞,WN 1802 B是从一只18月龄BALB/c小鼠脾脏中分离出的一种天然存在的小鼠白血病病毒。正常脾脏和骨髓细胞比从胸腺和淋巴结制备的细胞更易被感染。无胸腺裸鼠的脾细胞也能很容易地被病毒感染。允许性细胞不容易摄取铁屑,也不黏附于塑料。用脂多糖(一种骨髓来源的淋巴细胞促有丝分裂原)处理的脾脏和淋巴结细胞中,小鼠白血病病毒的外源性复制增强。相反,用胸腺来源的淋巴细胞促有丝分裂原植物血凝素和伴刀豆球蛋白A处理的细胞支持小鼠白血病病毒复制的能力较弱。这些研究表明,WN 1802 B的天然宿主是骨髓来源的淋巴细胞。