Pastore A, Carinci F, Pelucchi S
ORL Division, University of Ferrara, Italy.
Acta Otorhinolaryngol Ital. 2005 Jun;25(3):153-60.
Differentially expressed genes in various benign and malignant salivary gland tumours were identified by use of cDNA microarrays containing 19,000 human expressed sequence tags. Samples were derived from 5 patients with pleomorphic adenoma, 4 with Warthin's tumour, one with clear cell carcinoma, and 2 with mucoepidermoid carcinoma. Tumours were classified by using a subset of 486 genes. Benign Warthin's tumour and pleomorphic adenoma showed very distinctive gene expression patterns. A total of 133 genes differentiated the single malignant clear cell carcinoma from non-tumour salivary glands (p < 0.01), whereas only 16 genes separated it from the highly related benign pleomorphic adenoma (p < 0.01). A total of 57 cDNAs were associated with mucoepidermoid carcinoma (p < 0.01). The results show gene expression alterations common to all tumours and unique to each benign and malignant tumour. The numerous Expressed Sequence Tags of unknown function we identified could also become useful as tumour markers and represent a set of novel tumour-associated genes.
通过使用包含19,000个人类表达序列标签的cDNA微阵列，鉴定了各种良性和恶性涎腺肿瘤中的差异表达基因。样本取自5例多形性腺瘤患者、4例沃辛瘤患者、1例透明细胞癌患者和2例黏液表皮样癌患者。通过使用486个基因的子集对肿瘤进行分类。良性沃辛瘤和多形性腺瘤表现出非常独特的基因表达模式。共有133个基因将单一的恶性透明细胞癌与非肿瘤涎腺区分开来（p<0.01），而只有16个基因将其与高度相关的良性多形性腺瘤区分开来（p<0.01）。共有57个cDNA与黏液表皮样癌相关（p<0.01）。结果显示了所有肿瘤共有的基因表达改变以及每种良性和恶性肿瘤特有的基因表达改变。我们鉴定出的许多功能未知的表达序列标签也可能成为有用的肿瘤标志物，并代表一组新的肿瘤相关基因。
