Gan Xiang, Arita Kyoko, Isono Setsuko, Kitakawa Madoka, Yoshino Ken-ichi, Yonezawa Kazuyoshi, Kato Akihiro, Inoue Hirokazu, Isono Katsumi
Department of Biology, Faculty of Science, Kobe University, Kobe, Japan.
FEMS Microbiol Lett. 2006 Jan;254(1):157-64. doi: 10.1111/j.1574-6968.2005.00024.x.
The mitochondrial ribosome (mitoribosome) has highly evolved from its putative prokaryotic ancestor and varies considerably from one organism to another. To gain further insights into its structural and evolutionary characteristics, we have purified and identified individual mitochondrial ribosomal proteins of Neurospora crassa by mass spectrometry and compared them with those of the budding yeast Saccharomyces cerevisiae. Most of the mitochondrial ribosomal proteins of the two fungi are well conserved with each other, although the degree of conservation varies to a large extent. One of the N. crassa mitochondrial ribosomal proteins was found to be homologous to yeast Mhr1p that is involved in homologous DNA recombination and genome maintenance in yeast mitochondria.
线粒体核糖体（mitoribosome）与其假定的原核祖先相比已经高度进化，并且在不同生物体之间差异很大。为了进一步深入了解其结构和进化特征，我们通过质谱法纯化并鉴定了粗糙脉孢菌的单个线粒体核糖体蛋白，并将它们与芽殖酵母酿酒酵母的蛋白进行了比较。尽管保守程度在很大程度上有所不同，但这两种真菌的大多数线粒体核糖体蛋白彼此之间具有良好的保守性。发现粗糙脉孢菌的一种线粒体核糖体蛋白与酵母Mhr1p同源，后者参与酵母线粒体中的同源DNA重组和基因组维持。
