Amano H, Uemoto H, Kuroda K, Hosaka Y
Department of Microbiology, Osaka University of Pharmaceutical Sciences, Japan.
J Gen Virol. 1992 Aug;73 ( Pt 8):1969-75. doi: 10.1099/0022-1317-73-8-1969.
Using immunoelectron microscopy, the distribution of influenza A virus neuraminidase (NA) glycoproteins was examined, after performing immunoreactions to virions on the grid. With polyclonal antibody, the immunolabels of the glycoproteins were found to be homogeneously distributed, whereas with monoclonal antibody they were found to be distributed in clusters. After destruction of haemagglutinin (HA) but not of NA activity with a high concentration of trypsin, the remaining visible spikes were evenly distributed. This finding was consistent with the absence of immunolabelling with anti-HA antibody, and the homogeneous pattern of immunolabels with anti-NA polyclonal antibody, but not with the clustered labelling with the anti-NA monoclonal antibody. Thus, the immunolabelling image with anti-NA polyclonal antibody was considered to reflect the true one.
在网格上对病毒粒子进行免疫反应后，利用免疫电子显微镜检查甲型流感病毒神经氨酸酶（NA）糖蛋白的分布。使用多克隆抗体时，发现糖蛋白的免疫标记均匀分布，而使用单克隆抗体时，发现它们呈簇状分布。用高浓度胰蛋白酶破坏血凝素（HA）活性但不破坏NA活性后，剩余可见的刺突均匀分布。这一发现与抗HA抗体无免疫标记、抗NA多克隆抗体免疫标记呈均匀模式一致，但与抗NA单克隆抗体的簇状标记不一致。因此，抗NA多克隆抗体的免疫标记图像被认为反映了真实情况。
