Génie Microbiologique, Institut Pasteur, 28 rue du Docteur Roux, 75724 Paris Cedex 15, France.
EMBO J. 1988 Jan;7(1):231-7. doi: 10.1002/j.1460-2075.1988.tb02804.x.
We have identified an abundant ribonucleoprotein particle from Schizosaccharomyces pombe with properties related to those of the vertebrate signal recognition particle (SRP), including cytoplasmic localization, association with microsomes and ribosomes at low, but not high, salt concentrations and high resistance to micrococcal nuclease. The 256-nucleotide RNA component carries a 5'-triphosphate group and shows close secondary structure, and limited primary sequence homology to vertebrate 7SL RNA. 7SL-like RNAs were also detected in a number of other fungi. The single copy gene (SRP7) encoding S.pombe 7SL was disrupted by insertion of a transposon carrying the selective marker LEU2, and the disrupted gene was used to replace one chromosomal SRP7 gene in a diploid strain. Haploid srp7[unk] strains fail to germinate.
我们从裂殖酵母中鉴定出一种丰富的核糖核蛋白颗粒，其性质与脊椎动物信号识别颗粒（SRP）有关，包括细胞质定位、在低盐但不在高盐浓度下与微粒体和核糖体结合以及对微球菌核酸酶的高度抗性。256 个核苷酸的 RNA 成分带有 5'-三磷酸基团，并显示出紧密的二级结构，与脊椎动物 7SL RNA 的有限一级序列同源性。在许多其他真菌中也检测到类似 7SL 的 RNA。通过插入携带选择性标记 LEU2 的转座子来破坏编码裂殖酵母 7SL 的单拷贝基因（SRP7），并将该破坏的基因用于替换二倍体菌株中的一个染色体 SRP7 基因。单倍体 srp7[unk]菌株无法发芽。
