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粟酒裂殖酵母7SL RNA的遗传分析：一个包含保守四核苷酸环的结构基序对功能很重要。

Genetic analysis of Schizosaccharomyces pombe 7SL RNA: a structural motif that includes a conserved tetranucleotide loop is important for function.

作者信息

Liao X B, Brennwald P, Wise J A

机构信息

Department of Biochemistry, University of Illinois, Urbana 61801.

出版信息

Proc Natl Acad Sci U S A. 1989 Jun;86(11):4137-41. doi: 10.1073/pnas.86.11.4137.

DOI:10.1073/pnas.86.11.4137
PMID:2657742
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC287404/
Abstract

We have studied the effects of mutations in a 6-base segment of Schizosaccharomyces pombe 7SL RNA, which lies within a 35-nucleotide domain whose sequence and secondary structure are conserved in RNAs from many divergent organisms, including the 7SL component of human signal recognition particle (SRP). Surprisingly, many changes in this region can be tolerated under normal growth conditions. An exception is the lethality of several mutations at positions 159 and 160, 2 nucleotides previously shown to be protected from RNase digestion by the 19-kDa canine SRP protein. Nucleotide 160 is, in addition, the most highly conserved base in a consensus sequence for the most common tetranucleotide loop in ribosomal RNAs. Mutations that are likely to affect the stability and/or conformation of the RNA give rise to a conditional phenotype: when osmolarity of the medium is raised, the RNAs become partially or completely defective in function at high temperature.

摘要

我们研究了粟酒裂殖酵母7SL RNA中一个6碱基片段的突变效应，该片段位于一个35核苷酸结构域内，其序列和二级结构在许多不同生物的RNA中保守，包括人类信号识别颗粒（SRP）的7SL组分。令人惊讶的是，该区域的许多变化在正常生长条件下可以耐受。一个例外是159和160位的几个突变具有致死性，这两个核苷酸先前已显示受到19-kDa犬SRP蛋白的保护而免受RNase消化。此外，核苷酸160是核糖体RNA中最常见四核苷酸环共有序列中保守性最高的碱基。可能影响RNA稳定性和/或构象的突变产生条件性表型：当培养基渗透压升高时，RNA在高温下功能部分或完全缺陷。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6c4/287404/6be7f80253d2/pnas00251-0220-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6c4/287404/0de010fe5f2d/pnas00251-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6c4/287404/851d86d104e6/pnas00251-0219-b.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6c4/287404/6b2769d9934d/pnas00251-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6c4/287404/657a243b6daa/pnas00251-0220-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6c4/287404/e95543d5dca4/pnas00251-0220-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6c4/287404/6be7f80253d2/pnas00251-0220-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6c4/287404/0de010fe5f2d/pnas00251-0219-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6c4/287404/851d86d104e6/pnas00251-0219-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6c4/287404/e812d8d3c23a/pnas00251-0219-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6c4/287404/23a6c6401d87/pnas00251-0219-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6c4/287404/6b2769d9934d/pnas00251-0220-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6c4/287404/657a243b6daa/pnas00251-0220-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6c4/287404/e95543d5dca4/pnas00251-0220-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d6c4/287404/6be7f80253d2/pnas00251-0220-d.jpg

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本文引用的文献

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7SL RNA from Schizosaccharomyces pombe is encoded by a single copy essential gene.裂殖酵母的 7SL RNA 由一个单拷贝必需基因编码。
EMBO J. 1988 Jan;7(1):231-7. doi: 10.1002/j.1460-2075.1988.tb02804.x.
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Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.内质网蛋白质转运所需的膜相关蛋白质复合物的纯化。
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Disassembly and reconstitution of signal recognition particle.信号识别颗粒的拆卸与重组
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Genetic and biochemical analysis of the fission yeast ribonucleoprotein particle containing a homolog of Srp54p.对含有Srp54p同源物的裂殖酵母核糖核蛋白颗粒的遗传和生化分析。
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