Interactions Moléculaires Génomiques, Laboratoire associé au Centre National de la Recherche Scientifique, Université Paris-Sud, Bât, 400 F-91405 Orsay Cedex, France.
EMBO J. 1988 Jan;7(1):253-9. doi: 10.1002/j.1460-2075.1988.tb02807.x.
The initiation of hybrid DNA in the b2 spore colour gene was investigated by the analysis of non-Mendelian segregation asci in the progeny of crosses involving several allelic b2 mutations. These analyses showed that, instead of the unique initiation region of hybrid DNA in b2, assumed previously by Paquette and Rossignol (1978), there are multiple sites for hybrid DNA starting. They produce major and minor fractions of hybrid DNA. The major fraction starts upstream of the left end of the gene, propagates rightwards and can cover its entire length. The minor fractions do not span the left b2 end. One of them is detected within the left half of the gene, the others within its right half.
通过分析涉及几个等位基因突变的杂交后代中的非孟德尔分离子囊,研究了 b2 孢子颜色基因中杂种 DNA 的起始。这些分析表明,在 b2 中,杂种 DNA 的起始区域不是以前由 Paquette 和 Rossignol(1978)假设的独特区域,而是有多个杂种 DNA 起始的位点。它们产生杂种 DNA 的主要和次要部分。主要部分从基因左端上游开始,向右传播,可以覆盖其整个长度。次要部分不跨越基因的左 b2 端。其中一个在基因的左半部分检测到,其他的在其右半部分检测到。