一种用于检测黄嘌呤氧化酶抑制剂和超氧化物清除剂的快速薄层色谱自显影法。
A rapid TLC autographic method for the detection of xanthine oxidase inhibitors and superoxide scavengers.
作者信息
Ramallo I Ayelen, Zacchino Susana A, Furlan Ricardo L E
机构信息
Cátedra de Farmacognosia, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, S2002LRK Rosario, Argentina.
出版信息
Phytochem Anal. 2006 Jan-Feb;17(1):15-9. doi: 10.1002/pca.874.
A new bioautographic assay suitable for the localization of xanthine oxidase inhibitors and superoxide radical scavengers present in a complex matrix is described. Enzyme activity is detected by reaction of superoxide radicals with nitroblue tetrazolium to form a blue formazan salt. Both activities can be differentiated using a non-enzymatic version of the autographic assay wherein superoxide is chemically generated.
本文描述了一种新的生物自显影测定法，适用于定位复杂基质中存在的黄嘌呤氧化酶抑制剂和超氧自由基清除剂。通过超氧自由基与硝基蓝四唑反应形成蓝色甲臜盐来检测酶活性。使用自显影测定法的非酶版本可以区分这两种活性，其中超氧是化学产生的。
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