Morgan R E, Westwood N J
School of Chemistry, Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews, Fife, KY16 9ST, UK.
Parasitology. 2004;128 Suppl 1:S71-9. doi: 10.1017/S0031182004007073.
High throughput technologies continue to develop in response to the challenges set by the genome projects. This article discusses how the techniques of both high throughput screening (HTS) and synthesis can influence research in parasitology. Examples of the use of targeted and phenotype-based HTS using unbiased compound collections are provided. The important issue of identifying the protein target(s) of bioactive compounds is discussed from the synthetic chemist's perspective. This article concludes by reviewing recent examples of successful target identification studies in parasitology.
为应对基因组计划带来的挑战，高通量技术不断发展。本文讨论了高通量筛选（HTS）技术和合成技术如何影响寄生虫学研究。文中提供了使用无偏差化合物库进行靶向和基于表型的高通量筛选的实例。从合成化学家的角度讨论了鉴定生物活性化合物蛋白质靶点这一重要问题。本文通过回顾寄生虫学中成功的靶点鉴定研究的近期实例来作总结。
