Mahajan Shweta, Garg Angad, Goel Mayurika, Kumar P, Gupta K C
Nucleic Acids Research Laboratory, Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, Delhi 110 007, India.
Anal Biochem. 2006 Apr 15;351(2):273-81. doi: 10.1016/j.ab.2005.12.027. Epub 2006 Jan 17.
A universal reagent 1-O-(4,4'-dimethoxytrityl)-6-aminohexanol (DTAH) is described for the estimation of surface-bound functionalities (epoxy, aldehyde, and carboxyl) required for preparation of oligonucleotide arrays (biochips). The method involves the reaction of universal reagent DTAH with surface-bound functionality under microwaves for 10 min, followed by washings to remove the excess reagent. In the subsequent step, a weighed amount of DTAH-treated surface is exposed to acid to liberate 4,4'-dimethoxytrityl cation, which is measured at 505 nm to determine the functional group loading on the surface.
描述了一种通用试剂1-O-(4,4'-二甲氧基三苯甲基)-6-氨基己醇(DTAH)，用于评估制备寡核苷酸阵列(生物芯片)所需的表面结合官能团(环氧基、醛基和羧基)。该方法包括将通用试剂DTAH与表面结合官能团在微波下反应10分钟，然后洗涤以除去过量的试剂。在随后的步骤中，将称量好的经DTAH处理的表面暴露于酸中以释放4,4'-二甲氧基三苯甲基阳离子，在505nm处测量该阳离子以确定表面上官能团的负载量。
