Aldai N, Osoro K, Barrón L J R, Nájera A I
Area de Sistemas de Producción Animal, Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Apdo. 13, 33300 Villaviciosa, Asturias, Spain.
J Chromatogr A. 2006 Mar 31;1110(1-2):133-9. doi: 10.1016/j.chroma.2006.01.049. Epub 2006 Feb 7.
The optimisation and validation of a gas-liquid chromatographic (GLC) method using direct saponification with KOH/methanol followed by a derivatization with (trimethylsilyl)diazomethane was carried out trying to overcome all the difficulties posed by the analysis of complex mixtures of fatty acids (FAs) in animal fat tissues. The presented method allowed sensitive, selective and simultaneous determination of a wide range of different FAs, including short-chain FAs, branched-chain FAs and conjugated linoleic acid isomers in the same GLC run along with other well known saturated, monounsaturated and polyunsaturated FAs. To demonstrate the feasibility of the procedure, the total FA profile of beef meat was characterised.
采用KOH/甲醇直接皂化，随后用（三甲基硅基）重氮甲烷进行衍生化，对气液色谱（GLC）方法进行了优化和验证，旨在克服动物脂肪组织中脂肪酸（FAs）复杂混合物分析所带来的所有困难。所提出的方法能够灵敏、选择性地同时测定多种不同的脂肪酸，包括短链脂肪酸、支链脂肪酸和共轭亚油酸异构体，在同一GLC运行中，还能同时测定其他众所周知的饱和、单不饱和和多不饱和脂肪酸。为证明该方法的可行性，对牛肉的总脂肪酸谱进行了表征。
