Kaplan M A, Hays R M, Blumenfeld O O
J Membr Biol. 1975;20(1-2):181-90. doi: 10.1007/BF01870635.
Previous studies have shown that urea and acetamide traverse the erythrocyte membrane by way of facilitated diffusion. The nature of this selective pathway is unknown. The present studies investigate the effects of proteolytic enzymes and crosslinking agents on amide transport. Cleavage of the erythrocyte membrane surface by pronase or trypsin had no effect on urea and acetamide permeability or inhibition by phloretin. These findings suggest that the sialoglycopeptide segment of the sialoglycoproteins is not critical to urea and acetamide transport. In addition, extensive crosslinking of membrane proteins with glutaraldehyde had no effect on amide transport in the absence or presence of phloretin.
先前的研究表明，尿素和乙酰胺通过易化扩散穿过红细胞膜。这种选择性途径的性质尚不清楚。目前的研究调查了蛋白水解酶和交联剂对酰胺转运的影响。链霉蛋白酶或胰蛋白酶对红细胞膜表面的切割对尿素和乙酰胺的通透性或根皮素的抑制作用没有影响。这些发现表明，唾液糖蛋白的唾液糖肽段对尿素和乙酰胺的转运并不关键。此外，在不存在或存在根皮素的情况下，用戊二醛对膜蛋白进行广泛交联对酰胺转运没有影响。
